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. 1964 Dec;88(6):1612–1617. doi: 10.1128/jb.88.6.1612-1617.1964

DEPENDENCE OF THE CONTENT OF CELL ENVELOPES ON THE GROWTH RATE OF BACILLUS MEGATERIUM

I J Sud 1, M Schaechter 1
PMCID: PMC277462  PMID: 14240947

Abstract

Sud, I. J. (Tufts University School of Medicine, Boston, Mass.), and M. Schaechter. Dependence of the content of cell envelopes on the growth rate of Bacillus megaterium. J. Bacteriol. 88:1612–1617. 1964.—The amount of cell-wall and cell-membrane material was measured in Bacillus megaterium growing at different steady-state rates. On a dry weight basis, the content of wall hexosamine and diaminopimelic acid, and of membrane lipid phosphorus was found to be inversely proportional to the rate of growth. During the transition from slow to fast growth, the synthesis of envelope materials showed a lag of 20 to 30 min. Possible implications of these findings are discussed in terms of regulatory mechanism.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DRYER R. L., TAMMES A. R., ROUTH J. I. The determination of phosphorus and phosphatase with N-phenyl-p-phenylenediamine. J Biol Chem. 1957 Mar;225(1):177–183. [PubMed] [Google Scholar]
  2. ECKER R. E., SCHAECHTER M. RIBOSOME CONTENT AND THE RATE OF GROWTH OF SALMONELLA TYPHIMURIUM. Biochim Biophys Acta. 1963 Oct 15;76:275–279. [PubMed] [Google Scholar]
  3. HANCOCK R., PARK J. T. Cell-wall synthesis by Staphylococcus aureus in the presence of chloramphenicol. Nature. 1958 Apr 12;181(4615):1050–1052. doi: 10.1038/1811050a0. [DOI] [PubMed] [Google Scholar]
  4. KJELDGAARD N. O., MAALOE O., SCHAECHTER M. The transition between different physiological states during balanced growth of Salmonella typhimurium. J Gen Microbiol. 1958 Dec;19(3):607–616. doi: 10.1099/00221287-19-3-607. [DOI] [PubMed] [Google Scholar]
  5. KOLB J. J., WEIDNER M. A., TOENNIES G. Microdetermination of lipid phosphorus as a measure of bacterial membrane substance. Anal Biochem. 1963 Jan;5:78–82. doi: 10.1016/0003-2697(63)90061-7. [DOI] [PubMed] [Google Scholar]
  6. MANDELSTAM J., ROGERS H. J. Chloramphenicol-resistant incorporation of amino-acids into Staphylococci and cell-wall synthesis. Nature. 1958 Apr 5;181(4614):956–957. doi: 10.1038/181956a0. [DOI] [PubMed] [Google Scholar]
  7. NEIDHARDT F. C. EFFECTS OF ENVIRONMENT ON THE COMPOSITION OF BACTERIAL CELLS. Annu Rev Microbiol. 1963;17:61–86. doi: 10.1146/annurev.mi.17.100163.000425. [DOI] [PubMed] [Google Scholar]
  8. NEIDHARDT F. C., MAGASANIK B. Studies on the role of ribonucleic acid in the growth of bacteria. Biochim Biophys Acta. 1960 Jul 29;42:99–116. doi: 10.1016/0006-3002(60)90757-5. [DOI] [PubMed] [Google Scholar]
  9. PARK J. T., HANCOCK R. A fractionation procedure for studies of the synthesis of cell-wall mucopeptide and of other polymers in cells of Staphylococcus aureus. J Gen Microbiol. 1960 Feb;22:249–258. doi: 10.1099/00221287-22-1-249. [DOI] [PubMed] [Google Scholar]
  10. SALTON M. R., PAVLIK J. G. Studies of the bacterial cell wall. VI. Wall composition and sensitivity to lysozyme. Biochim Biophys Acta. 1960 Apr 22;39:398–407. doi: 10.1016/0006-3002(60)90191-8. [DOI] [PubMed] [Google Scholar]
  11. TOENNIES G., SHOCKMAN G. D., KOLB J. J. Differential effects of amino acid deficiencies on bacterial cytochemistry. Biochemistry. 1963 Mar-Apr;2:294–296. doi: 10.1021/bi00902a017. [DOI] [PubMed] [Google Scholar]
  12. WEIBULL C., BERGSTROM L. The chemical nature of the cytoplasmic membrane and cell wall of Bacillus megaterium, strain M. Biochim Biophys Acta. 1958 Nov;30(2):340–351. doi: 10.1016/0006-3002(58)90059-3. [DOI] [PubMed] [Google Scholar]

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