Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1964 Dec;88(6):1672–1677. doi: 10.1128/jb.88.6.1672-1677.1964

SPECIFICITY OF IMPROVED METHODS FOR MYCOBACTIN BIOASSAY BY ARTHROBACTER TERREGENS

Alan D Antoine 1, Norman E Morrison 1, John H Hanks 1
PMCID: PMC277472  PMID: 14240956

Abstract

Antoine, Alan D. (Johns Hopkins University-Leonard Wood Memorial Leprosy Research Laboratory, Baltimore, Md.), Norman E. Morrison, and John H. Hanks. Specificity of improved methods for mycobactin bioassay by Arthrobacter terregens. J. Bacteriol. 88:1672–1677. 1964.—Arthrobacter terregens was used to assay mycobactin, a growth factor for Mycobacterium paratuberculosis. Improved techniques permit the assay of mycobactin within 3 to 4 days by agarplate or liquid-medium methods. For the agarplate method, Arthrobacter terregens gave linear increases in zonal growth at mycobactin concentrations of 0.07 to 0.30 μg per spot; for the liquid-medium method, linear increases in turbidimetric growth occurred at 0.05 to 0.27 μg/ml. Specificity studies show that the mycobactin hydrolytic products, cobactin and mycobactic acid, function as growth stimulators, but the high concentrations required would produce only minimal interference in mycobactin assays. Furthermore, the response to mycobactic acid is characterized by a delayed response of 3 days. Various synthetic hydroxylamine-containing compounds and metalchelating agents cannot replace the biological activity of mycobactin. Diacetylmycobactin is 7.4 times more effective than mycobactin as a growth stimulator.

Full text

PDF
1672

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURNHAM B. F., NEILANDS J. B. Studies on the metabolic function of the ferrichrome compounds. J Biol Chem. 1961 Feb;236:554–559. [PubMed] [Google Scholar]
  2. EL HAWARY M. F. S., THOMPSON R. H. S. Separation and estimation of blood keto acids by paper chromatography. Biochem J. 1953 Feb;53(3):340–347. doi: 10.1042/bj0530340. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. FRANCIS J., MACTURK H. M., MADINAVEITIA J., SNOW G. A. Mycobactin, a growth factor for Mycobacterium johnei. I. Isolation from Mycobacterium phlei. Biochem J. 1953 Nov;55(4):596–607. doi: 10.1042/bj0550596. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. LOCHHEAD A. G. Soil bacteria and growth-promoting substances. Bacteriol Rev. 1958 Sep;22(3):145–153. doi: 10.1128/br.22.3.145-153.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. NEILANDS J. B. Some aspects of microbial iron metabolism. Bacteriol Rev. 1957 Jun;21(2):101–111. doi: 10.1128/br.21.2.101-111.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. REICH C. V., HANKS J. H. USE OF ARTHROBACTER TERREGENS FOR BIOASSAY OF MYCOBACTIN. J Bacteriol. 1964 Jun;87:1317–1320. doi: 10.1128/jb.87.6.1317-1320.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES