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. 1965 Mar;89(3):583–588. doi: 10.1128/jb.89.3.583-588.1965

Effect of Partially Purified Streptococcal M Protein on the In Vitro Phagocytosis of Streptococcus pyogenes1

Gerald J Domingue a,2, William A Pierce Jr a
PMCID: PMC277506  PMID: 14275650

Abstract

Domingue, Gerald J. (Tulane University, New Orleans, La.), and William A. Pierce, Jr. Effect of partially purified streptococcal M protein on the in vitro phagocytosis of Streptococcus pyogenes. J. Bacteriol. 89:583–588. 1965.—M protein from Streptococcus pyogenes strain Bailey type 12 was adsorbed onto cells of a glossy mutant of this strain. Phagocytosis experiments were performed in vitro with rabbit peritoneal polymorphonuclear leukocytes. Direct microscopic counts suggested M protein-treated cells were more resistant to phagocytosis than were untreated cells. With the Cohn and Morse technique for evaluating quantitatively the fate of bacteria during phagocytosis, there was observed a more rapid drop in total and extracellular counts of treated, as compared with untreated, cells. Experiments with radioactively labeled streptococci showed that M-treated cells were more readily destroyed within leukocytes than were nontreated glossy streptococci, which suggested a reason for the scarcity of treated streptococci in direct microscopic counts.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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