Abstract
Low, Iolanda E. (Harvard Medical School, Boston, Mass.), and Monroe D. Eaton. Replication of Mycoplasma pneumoniae in broth culture. J. Bacteriol. 89:725–728. 1965.—Reproducible growth curves of Mycoplasma pneumoniae can be obtained with the use of shaking cultures, which allows earlier and higher yields of the organism than stationary or roller cultures. Decreasing oxygen tension clearly decreases the growth of M. pneumoniae to barely detectable levels, with or without glucose or pyruvate as substrates. The use of phenol red as a pH indicator in the medium is helpful in judging the replication of a culture so that log-phase organisms can be harvested in large amounts.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- CHANOCK R. M., HAYFLICK L., BARILE M. F. Growth on artificial medium of an agent associated with atypical pneumonia and its identification as a PPLO. Proc Natl Acad Sci U S A. 1962 Jan 15;48:41–49. doi: 10.1073/pnas.48.1.41. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CHANOCK R. M., JAMES W. D., FOX H. H., TURNER H. C., MUFSON M. A., HAYFLICK L. Growth of Eaton PPLO in broth and preparation of complement fixing antigen. Proc Soc Exp Biol Med. 1962 Aug-Sep;110:884–889. doi: 10.3181/00379727-110-27681. [DOI] [PubMed] [Google Scholar]
- CLYDE W. A., Jr Hemolysis in identifying Eaton's pleuro-pneumonia-like organism. Science. 1963 Jan 4;139(3549):55–55. doi: 10.1126/science.139.3549.55. [DOI] [PubMed] [Google Scholar]
- RODWELL A. W., RODWELL E. S. The breakdown of carbohydrates by Asterococcus mycoides, the organism of bovine pleuropneumonia. Aust J Biol Sci. 1954 Feb;7(1):18–30. [PubMed] [Google Scholar]
- RODWELL A. W., RODWELL E. S. The breakdown of pyruvate by Asterococcus mycoides, the organism of bovine pleuropneumonia. Aust J Biol Sci. 1954 Feb;7(1):31–36. [PubMed] [Google Scholar]
- RODWELL A. W., RODWELL E. S. The pathway for glucose oxidation by Asterococcus mycoides, the organism of bovine pleuropneumonia. Aust J Biol Sci. 1954 Feb;7(1):37–46. [PubMed] [Google Scholar]
- SOMERSON N. L., TAYLOR-ROBINSON D., CHANOCK R. M. Hemolyin production as an aid in the identification and quantitation of Eaton agent (Mycoplasma pneumoniae). Am J Hyg. 1963 Jan;77:122–128. doi: 10.1093/oxfordjournals.aje.a120290. [DOI] [PubMed] [Google Scholar]