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. 1965 Mar;89(3):734–739. doi: 10.1128/jb.89.3.734-739.1965

Preparation of Fluorescein Isothiocyanate-Labeled γ-Globulin by Dialysis, Gel Filtration, and Ion-Exchange Chromatography in Combination

Robert E Dedmon a,1, Albert W Holmes a, Friedrich Deinhardt a
PMCID: PMC277530  PMID: 14273654

Abstract

Dedmon, Robert E. (Presbyterian-St. Luke's Hospital, Chicago, Ill.), Albert W. Holmes, and Friedrich Deinhardt. Preparation of fluorescein isothiocyanate-labeled γ-globulin by dialysis, gel filtration, and ion-exchange chromatography in combination. J. Bacteriol. 89:734–739. 1965.—Antiviral immune γ-globulins isolated from rabbit and guinea pig sera were labeled through dialysis membranes with fluorescein isothiocyanate and purified in several ways to eliminate nonspecific staining. Gel filtration of the conjugate with Sephadex G-25 coarse beads followed by column fractionation with diethylaminoethyl-Sephadex yielded consistently highly specific staining materials. Fluorescein-protein ratios varied between 1.0 and 4.0. This technique has proved to be simple and reliable, and is less time-consuming than previous techniques.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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