Abstract
Porter, Billie M. (Communicable Disease Center, Kansas City, Kan.), Barbara K. Comfort, Robert W. Menges, Robert T. Habermann, and Coy D. Smith. Correlation of fluorescent antibody, histopathology, and culture on tissues from 372 animals examined for histoplasmosis and blastomycosis. J. Bacteriol. 89:748–751. 1965.—In a survey in the midwestern United States, tissues from 372 animals including 16 species were examined for Histoplasma capsulatum and Blastomyces dermatitidis. All of the specimens were cultured and studied by use of the fluorescent-antibody (FA) technique, and histopathological studies of tissue sections were done. Among the 372 animals, 300 were negative by all three methods of examination, 34 had blastomycosis, and 21 had histoplasmosis. The remaining 17 were positive for histoplasmosis with the FA technique but were negative by histopathology and culture. An animal was considered to have histoplasmosis or blastomycosis only when the specific fungus was isolated or demonstrated in tissue sections or exudates from cutaneous lesions. Of the 34 blastomycosis cases, 25 (74%) were positive by FA, 32 (94%) by histopathology, and 11 (32%) by culture. Of the 21 histoplasmosis cases, 15 (71%) were positive by FA, 16 (76%) by histopathology, and 14 (67%) by culture. It appeared that FA, histopathology, or culture alone was insufficient for a diagnosis. All three techniques should be employed for the most satisfactory diagnostic results.
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Selected References
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- GOLDMAN M., MOODY M. D., THOMASON B. M. Staining bacterial smears with fluorescent antibody. I. General methods for Malleomyces pseudomallei. J Bacteriol. 1956 Sep;72(3):357–361. doi: 10.1128/jb.72.3.357-361.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GORDON M. A. Fluorescent staining of Histoplasma capsulatum. J Bacteriol. 1959 Jun;77(6):678–681. doi: 10.1128/jb.77.6.678-681.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LYNCH H. J., Jr, PLEXICO K. L. A rapid method for screening sputums for Histoplasma capsulatum employing the fluorescent-antibody technic. N Engl J Med. 1962 Apr 19;266:811–814. doi: 10.1056/NEJM196204192661606. [DOI] [PubMed] [Google Scholar]
- MARSHALL J. D., EVELAND W. C., SMITH C. W. Superiority of fluorescein isothiocyanate (Riggs) for fluorescent-antibody technic with a modification of its application. Proc Soc Exp Biol Med. 1958 Aug-Sep;98(4):898–900. doi: 10.3181/00379727-98-24222. [DOI] [PubMed] [Google Scholar]
- RIGGS J. L., SEIWALD R. J., BURCKHALTER J. H., DOWNS C. M., METCALF T. G. Isothiocyanate compounds as fluorescent labeling agents for immune serum. Am J Pathol. 1958 Nov-Dec;34(6):1081–1097. [PMC free article] [PubMed] [Google Scholar]