Abstract
Arm, H. G. (Naval Medical Research Institute, Bethesda, Md.), T. M. Floyd, J. E. Faber, and J. R. Hayes. Use of ligated segments of rabbit small intestine in experimental shigellosis. J. Bacteriol. 89:803–809. 1965.—Inoculation of ligated segments of small intestine in living rabbits with broth cultures or resting-cell suspensions of recently isolated strains of Shigella caused distension of the segments by accumulation of exudate within 12 hr. Histological changes characteristic of an inflammatory response were similar to those of human bacillary dysentery. Tissue response and accumulation of exudate preceded demonstrable increase in numbers of shigellae inoculated as 2 × 1010 resting cells. Capability of shigellae to provoke intestinal response was not related to any particular serological group. The active principles concerned with eliciting intestinal response were associated only with preparations containing living organisms. Ability of recently isolated strains to elicit response diminished rapidly during culture on artificial media. The capability was preserved indefinitely by lyophilization soon after isolation from acute bacillary dysentery infections of man. Advantages of using shigellae recently isolated for investigating possible mechanisms of pathogenesis were indicated. During the summer months, the rabbit small intestine was refractory to the activity of shigellae, and positive responses were not observed. Use of ligated segments of rabbit small intestine qualified as an indicator of virulence for the rabbit; and, virulence for the rabbit showed a high degree of correlation with a short period of culture of shigellae on artificial media after isolation from human bacillary dysentery infections.
Full text
PDF![803](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8185/277540/1c2dec8cd10d/jbacter00432-0289.png)
![804](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8185/277540/6c88502b2448/jbacter00432-0290.png)
![805](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8185/277540/9bacb555e7e0/jbacter00432-0291.png)
![806](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8185/277540/f6c9b25ed8f2/jbacter00432-0292.png)
![807](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8185/277540/68f894446c6f/jbacter00432-0293.png)
![808](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8185/277540/f902943eff6a/jbacter00432-0294.png)
![809](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8185/277540/f992ff043cb3/jbacter00432-0295.png)
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- DE S. N., BHATTACHARYA K., SARKAR J. K. A study of the pathogenicity of strains of Bacterium coli from acute and chronic enteritis. J Pathol Bacteriol. 1956 Jan;71(1):201–209. doi: 10.1002/path.1700710126. [DOI] [PubMed] [Google Scholar]
- DE S. N., CHATTERJE D. N. An experimental study of the mechanism of action of Vibriod cholerae on the intestinal mucous membrane. J Pathol Bacteriol. 1953 Oct;66(2):559–562. doi: 10.1002/path.1700660228. [DOI] [PubMed] [Google Scholar]
- FORMAL S. B., ABRAMS G. D., SCHNEIDER H., SPRINZ H. Experimental Shigella infections. VI. Role of the small intestine in an experimental infection in guinea pigs. J Bacteriol. 1963 Jan;85:119–125. doi: 10.1128/jb.85.1.119-125.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FORMAL S. B., DAMMIN G. J., LABREC E. H., SCHNEIDER H. Experimental Shigella infections: characteristics of a fatal infection produced in guinea pigs. J Bacteriol. 1958 May;75(5):604–610. doi: 10.1128/jb.75.5.604-610.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FORMAL S. B., DAMMIN G. J., SCHNEIDER H., LABREC E. H. Experimental Shigella infections. II. Characteristics of a fatal enteric infection in guinea pigs following the subcutaneous inoculation of carbon tetrachloride. J Bacteriol. 1959 Dec;78:800–804. doi: 10.1128/jb.78.6.800-804.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FORMAL S. B., DAMMIN G., SPRINZ H., KUNDEL D., SCHNEIDER H., HOROWITZ R. E., FORBES M. Experimental Shigella infections. V. Studies in germ-free guinea pigs. J Bacteriol. 1961 Aug;82:284–287. doi: 10.1128/jb.82.2.284-287.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FORMAL S. B., KUNDEL D., SCHNEIDER H., KUNEVN, SPRINZ H. Studies with Vibrio cholerae in the ligated loop of the rabbit intestine. Br J Exp Pathol. 1961 Oct;42:504–510. [PMC free article] [PubMed] [Google Scholar]
- FRETER R. Experimental enteric Shigella and Vibrio infections in mice and guinea pigs. J Exp Med. 1956 Sep 1;104(3):411–418. doi: 10.1084/jem.104.3.411. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JENKIN C. R., ROWLEY D. Possible factors in the pathogenesis of cholera. Br J Exp Pathol. 1959 Oct;40:474–481. [PMC free article] [PubMed] [Google Scholar]
- LABREC E. H., FORMAL S. B. Experimental Shigella infections. IV. Fluorescent antibody studies of an infection in guinea pigs. J Immunol. 1961 Nov;87:562–572. [PubMed] [Google Scholar]
- McNAUGHT W., ROBERTS G. B. Enteropathogenic effects of strains of Bacterium coli isolated from cases of gastro-enteritis. J Pathol Bacteriol. 1958 Jul;76(1):155–158. doi: 10.1002/path.1700760117. [DOI] [PubMed] [Google Scholar]
- TAYLOR J., MALTBY M. P., PAYNE J. M. Factors influencing the response of ligated rabbit-gut segments to injected Escherichia coli. J Pathol Bacteriol. 1958 Oct;76(2):491–499. doi: 10.1002/path.1700760218. [DOI] [PubMed] [Google Scholar]
- WEBSTER M. E., SAGIN J. F., LANDY M., JOHNSON A. G. Studies on the O antigen of Salmonella typhosa. I. Purification of the antigen. J Immunol. 1955 Jun;74(6):455–465. [PubMed] [Google Scholar]