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. 1965 Mar;89(3):813–818. doi: 10.1128/jb.89.3.813-818.1965

Hemolysin of Mycoplasma pneumoniae

Norman L Somerson a, Robert H Purcell a, David Taylor-Robinson a,1, Robert M Chanock a
PMCID: PMC277542  PMID: 14273666

Abstract

Somerson, Norman L. (National Institute of Allergy and Infectious Diseases, Bethesda, Md.), Robert H. Purcell, David Taylor-Robinson, and Robert M. Chanock. Hemolysin of Mycoplasma pneumoniae. J. Bacteriol. 89:813–818. 1965.—Discrete colonies of Mycoplasma pneumoniae or M. laidlawii on agar produced complete (β) lysis of guinea pig erythrocytes. Crowding of colonies on agar plates, omission of yeast extract from the medium, and incubation under reduced oxygen tension inhibited hemolysin production. Colonies which were not viable after ultraviolet radiation or heating at 56 C did not produce hemolysin. In addition, hemolysis was suppressed in old cultures in which viability was diminished, and was eliminated when colonies were removed from the agar surface by micromanipulation. The hemolysin passed through a viscose dialysis membrane. These findings suggest that the hemolysin is labile, continuously released by the colonies, of low molecular weight, and probably nonprotein in nature.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BERK R. S. Production and characterization of Pseudomon as aeruginosa hemolysin. J Bacteriol. 1962 Nov;84:1041–1048. doi: 10.1128/jb.84.5.1041-1048.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CHANOCK R. M., MUFSON M. A., SOMERSON N. L., COUCH R. B. ROLE OF MYCOPLASMA (PPLO) IN HUMAN RESPIRATORY DISEASE. Am Rev Respir Dis. 1963 Sep;88:SUPPL–239. doi: 10.1164/arrd.1963.88.3P2.218. [DOI] [PubMed] [Google Scholar]
  3. CLYDE W. A., Jr Hemolysis in identifying Eaton's pleuro-pneumonia-like organism. Science. 1963 Jan 4;139(3549):55–55. doi: 10.1126/science.139.3549.55. [DOI] [PubMed] [Google Scholar]
  4. EDWARD D. G. F. An investigation of the biological properties of organisms of the pleuropneumonia group, with suggestions regarding the identification of strains. J Gen Microbiol. 1950 Sep;4(3):311–329. doi: 10.1099/00221287-4-3-311. [DOI] [PubMed] [Google Scholar]
  5. GIRARD K. F., SBARRA A. J., BARDAWIL W. A. Serology of Listeria monocytogenes. I. Characteristics of the soluble hemolysin. J Bacteriol. 1963 Feb;85:349–355. doi: 10.1128/jb.85.2.349-355.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. JENKINS E. M., NJOKU-OBI A. N., ADAMS E. W. PURIFICATION OF THE SOLUBLE HEMOLYSINS OF LISTERIA MONOCYTOGENES. J Bacteriol. 1964 Aug;88:418–424. doi: 10.1128/jb.88.2.418-424.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LAMPEN J. O., GILL J. W., ARNOW P. M., MAGANA-PLAZA I. INHIBITION OF THE PLEUROPNEUMONIA-LIKE ORGANISM MYCOPLASMA GALLISEPTICUM BY CERTAIN POLYENE ANTIFUNGAL ANTIBIOTICS. J Bacteriol. 1963 Nov;86:945–949. doi: 10.1128/jb.86.5.945-949.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LIU C. Studies on primary atypical pneumonia. I. Localization, isolation, and cultivation of a virus in chick embryos. J Exp Med. 1957 Oct 1;106(4):455–466. doi: 10.1084/jem.106.4.455. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Macfarlane M. G., Knight B. C. The biochemistry of bacterial toxins: The lecithinase activity of Cl. welchii toxins. Biochem J. 1941 Sep;35(8-9):884–902. doi: 10.1042/bj0350884. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. RAZIN S., COHEN A. Nutritional requirements and metabolism of Mycoplasma laidlawii. J Gen Microbiol. 1963 Jan;30:141–154. doi: 10.1099/00221287-30-1-141. [DOI] [PubMed] [Google Scholar]
  12. SOMERSON N. L., TAYLOR-ROBINSON D., CHANOCK R. M. Hemolyin production as an aid in the identification and quantitation of Eaton agent (Mycoplasma pneumoniae). Am J Hyg. 1963 Jan;77:122–128. doi: 10.1093/oxfordjournals.aje.a120290. [DOI] [PubMed] [Google Scholar]
  13. TASMAN A., VAN DER SLOT J. On a new method of preparing staphylococcus toxin and toxoid. Antonie Van Leeuwenhoek. 1953;19(3):256–266. doi: 10.1007/BF02594852. [DOI] [PubMed] [Google Scholar]
  14. TAYLOR-ROBINSON D., SOMERSON N. L., TURNER H. C., CHANOCK R. M. SEROLOGICAL RELATIONSHIPS AMONG HUMAN MYCOPLASMAS AS SHOWN BY COMPLEMENT-FIXATION AND GEL DIFFUSION. J Bacteriol. 1963 Jun;85:1261–1273. doi: 10.1128/jb.85.6.1261-1273.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Warren J. Observations on Some Biological Characteristics of Organisms of the Pleuropneumonia Group. J Bacteriol. 1942 Feb;43(2):211–228. doi: 10.1128/jb.43.2.211-228.1942. [DOI] [PMC free article] [PubMed] [Google Scholar]

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