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. Author manuscript; available in PMC: 2009 Nov 10.
Published in final edited form as: Nature. 2008 Sep 24;455(7217):1244–1247. doi: 10.1038/nature07344

Figure 4.

Figure 4

GILT activates LLO by using the classical thioredoxin reduction mechanism. Wild type macrophages, GILT −/− macrophages, or GILT −/− macrophages reconstituted with the C71S GILT trapping mutant were uninfected or infected with either wild type L. monocytogenes or ΔLLO L. monocytogenes for 2 h prior to detergent solubilization, immunoprecipitation, and SDS-PAGE; (top panel), western blot of LLO in the detergent extracts; (middle panel), western blot with an LLO-specific antiserum of immunoprecipitates isolated from the extracts using a control mAb (left three lanes) or an anti GILT mAb (right nine lanes). LLO is only co-precipitated with the C71S trapping mutant; (bottom panel), western blot for GILT in the immunoprecipitates.