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. 1965 Apr;89(4):988–992. doi: 10.1128/jb.89.4.988-992.1965

Drug Resistance of Staphylococci II. Joint Elimination and Joint Transduction of the Determinants of Penicillinase Production and Resistance to Macrolide Antibiotics

Susumu Mitsuhashi 1, Hajime Hashimoto 1, Megumi Kono 1, Masato Morimura 1
PMCID: PMC277583  PMID: 14276126

Abstract

Mitsuhashi, Susumu (Gunma University, Maebashi, Japan), Hajime Hashimoto, Megumi Kono, and Masato Morimura. Drug resistance of staphylococci. II. Joint elimination and joint transduction of the determinants of penicillinase production and resistance to macrolide antibiotics. J. Bacteriol. 89:988–992. 1965.—Strains of Staphylococcus aureus, which show high resistance to macrolide antibiotics (erythromycin, oleandomycin, leucomycin, and spiramycin) and the capacity to produce penicillinase, have been isolated from clinical sources. The determinants of penicillinase production (PCase+) and resistance to macrolide antibiotics (MACr) of these strains were irreversibly eliminated by treatment with acridine or with ultraviolet light. Among the 18 strains tested, PCase+ and MACr were eliminated from all strains except one, which lost only PCase+ but not MACr. The characters PCase+ and MACr were jointly transduced with the aid of phage lysates, obtained from the resistant donors by ultraviolet irradiation, into staphylococcal strains sensitive to PC and MAC. Segregation of PCase+ and MACr was rarely observed after transduction. From these results, it is suggested that the determinants of both PCase+ and MACr of staphylococci are located close together on a single genetic element, i.e., a plasmid (or episome), which exists extrachromosomally.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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