Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1965 Apr;89(4):1020–1025. doi: 10.1128/jb.89.4.1020-1025.1965

Comparison of Lipids and Lipopolysaccharide from the Bacillary and L Forms of Proteus P18

J A Nesbitt III 1, W J Lennarz 1
PMCID: PMC277589  PMID: 14276089

Abstract

Nesbitt, J. A., III (The Johns Hopkins University School of Medicine, Baltimore, Md.), and W. J. Lennarz. Comparison of lipids and lipopolysaccharides from the bacillary and L forms of Proteus P18. J. Bacteriol. 89:1020–1025. 1965.—Comparative studies on the L form of Proteus P18 and the parent bacterium grown in a defined medium showed that the L form contained 1.5 times as much extractable lipid (dry weight) as the bacillary form. The composition of the lipids extractable by chloroform-methanol was quite similar in the two bacterial forms. The occurrence of myristate and β-hydroxymyristate in the bound, nonextractable lipid was found to be a reflection of the presence of lipopolysaccharide (LPS) in each organism. The bacillary organism contains three to four times as much LPS as the L form. The LPS isolated from both organisms contains heptose, hexosamine, phosphorus, 3-deoxyoctulosonate, glucose, galactose, and fatty acids.

Full text

PDF
1020

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ABRAMS R. Y. A method for the cultivation of L forms in liquid media. J Bacteriol. 1955 Aug;70(2):251–251. doi: 10.1128/jb.70.2.251-251.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BLOSS L. The relationship between two Proteus mirabilis L-forms and bacteriophages. Arch Mikrobiol. 1963;45:1–6. doi: 10.1007/BF00410292. [DOI] [PubMed] [Google Scholar]
  3. BURTON A. J., CARTER H. E. PURIFICATION AND CHARACTERIZATION OF THE LIPID A COMPONENT OF THE LIPOPOLYSACCHARIDES FROM ESCHERICHIA COLI. Biochemistry. 1964 Mar;3:411–418. doi: 10.1021/bi00891a018. [DOI] [PubMed] [Google Scholar]
  4. CHUNG A. E., LAW J. H. CYCLOPROPANE FATTY ACID SYNTHETASE: PARTIAL PURIFICATION AND PROPERTIES. Biochemistry. 1964 Jul;3:967–974. doi: 10.1021/bi00895a021. [DOI] [PubMed] [Google Scholar]
  5. CROOM J. A., MCNEILL J. J., TOVE S. B. BIOTIN DEFICIENCY AND THE FATTY ACIDS OF CERTAIN BIOTIN-REQUIRING BACTERIA. J Bacteriol. 1964 Aug;88:389–394. doi: 10.1128/jb.88.2.389-394.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. FLECK J. [Study of the growth, in liquid media, of a strain of Proteus and its L form]. C R Seances Soc Biol Fil. 1963;157:183–185. [PubMed] [Google Scholar]
  7. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  8. GOLDFINE H., BLOCH K. On the origin of unsaturated fatty acids in clostridia. J Biol Chem. 1961 Oct;236:2596–2601. [PubMed] [Google Scholar]
  9. KREMBEL J. ETUDE DES LIPIDES DE LA FORME L STABLE DE PROT'EUS P 18: IDENTIFICATION DES ACIDES GRAS DE LA FRACTION AC'ETONOSOLUBLE. Pathol Microbiol (Basel) 1963;26:592–600. [PubMed] [Google Scholar]
  10. KREMBEL J. ETUDE DES LIPIDES DE LA FORME L STABLE DE PROTEUS P 18: D'ETERMINATION DE LA STRUCTURE DES ACIDES INSATUR'ES ET IDENTIFICATION D'UN ACIDE 'A CYCLE PROPANIQUE. ETUDE DE L'HYDROSOLUBLE DE SAPONIFICATION DES GRAISSES NEUTRES. Bull Soc Chim Biol (Paris) 1964;46:503–508. [PubMed] [Google Scholar]
  11. King E. J. The colorimetric determination of phosphorus. Biochem J. 1932;26(2):292–297. doi: 10.1042/bj0260292. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. LENNARZ W. J. THE CHEMICAL CHARACTERIZATION AND ENZYMIC SYNTHESIS OF A BACTERIAL GLYCOLIPID. J Biol Chem. 1964 Sep;239:PC–3112. [PubMed] [Google Scholar]
  13. LIGHT R. J., LENNARZ W. J., BLOCH K. The metabolism of hydroxystearic acids in yeast. J Biol Chem. 1962 Jun;237:1793–1800. [PubMed] [Google Scholar]
  14. MANDEL P., SENSENBRENNER M., DE GREGORIO P., BADER A. M. Distribution of nucleic acids among different stable L forms of Proteus P 18. Nature. 1959 Aug 15;184(Suppl 8):566–566. doi: 10.1038/184566a0. [DOI] [PubMed] [Google Scholar]
  15. MINCK R., KIRN A. Pleuropneumonialike organisms and L forms of bacteria. Ann N Y Acad Sci. 1960 Jan 15;79:658–661. doi: 10.1111/j.1749-6632.1960.tb42738.x. [DOI] [PubMed] [Google Scholar]
  16. MORRISON T. H., WEIBULL C. The occurrence of cell wall constituents in stable Proteus L forms. Acta Pathol Microbiol Scand. 1962;55:475–482. doi: 10.1111/j.1699-0463.1962.tb04153.x. [DOI] [PubMed] [Google Scholar]
  17. OSBORN M. J., ROSEN S. M., ROTHFIELD L., ZELEZNICK L. D., HORECKER B. L. LIPOPOLYSACCHARIDE OF THE GRAM-NEGATIVE CELL WALL. Science. 1964 Aug 21;145(3634):783–789. doi: 10.1126/science.145.3634.783. [DOI] [PubMed] [Google Scholar]
  18. REBEL G., BADER-HIRSCH A. M., MANDEL P. RECHERCHES SUR LES LIPIDES DES FORMES L. D'ERIV'EES DU PROTEUS P 18. I. PR'ESENCE ET ABSORPTION DU CHOLEST'EROL. Bull Soc Chim Biol (Paris) 1963;45:1327–1343. [PubMed] [Google Scholar]
  19. REBEL G., BADER A. M., SENSENBRENNER M., MANDEL P. [Comparative study of the lipids of Proteus bacillus and a stable culture of derived L forms]. C R Hebd Seances Acad Sci. 1960 May 23;250:3516–3517. [PubMed] [Google Scholar]
  20. SMITH P. F. COMPARATIVE PHYSIOLOGY OF PLEUROPNEUMONIA-LIKE AND L-TYPE ORGANISMS. Bacteriol Rev. 1964 Jun;28:97–125. doi: 10.1128/br.28.2.97-125.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. SMITH P. F., ROTHBLAT G. H. Comparison of lipid composition of pleuropneumonia-like and L-type organisms. J Bacteriol. 1962 Mar;83:500–506. doi: 10.1128/jb.83.3.500-506.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. VENDRELY R., TULASNE R. Premières observations sur la constitution chimique des formes L des bactéries. Bull Soc Chim Biol (Paris) 1952;34(7-8):785–789. [PubMed] [Google Scholar]
  23. VON PRITTWITZ J., GAFFRON Das Verhalten eines Stammes von Proteus vulgaris und seiner L-Phase gegenüber hemologen Antiseren. Zentralbl Bakteriol Orig. 1955 Aug;163(5-6):313–318. [PubMed] [Google Scholar]
  24. WEIBULL C., BECKMAN H. Chemical and metabolic properties of various elements found in cultures of a stable Proteus L form. J Gen Microbiol. 1961 Mar;24:379–391. doi: 10.1099/00221287-24-3-379. [DOI] [PubMed] [Google Scholar]
  25. WHEELDON L. W., BRINLEY M., TURNER D. A. Simultaneous determination of glycerol, inositol, serine and ethanolamine in a phospholipid hydrolyzate. Anal Biochem. 1962 Dec;4:433–443. doi: 10.1016/0003-2697(62)90125-2. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES