Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1965 May;89(5):1186–1194. doi: 10.1128/jb.89.5.1186-1194.1965

Enzymatic Basis for d-Arabitol Production by Saccharomyces rouxii1

Jordan M Ingram a, W A Wood a
PMCID: PMC277626  PMID: 14292984

Abstract

Ingram, Jordan M. (Michigan State University, East Lansing), and W. A. Wood. Enzymatic basis for d-arabitol production by Saccharomyces rouxii. J. Bacteriol. 89:1186–1194. 1965.—The enzymatic steps in d-arabitol synthesis by Saccharomyces rouxii were studied. The fermentation of d-glucose-6-C14 gave rise to d-arabitol labeled at C-5; d-ribose of ribonucleic acid had the same isotope pattern. Crude extracts were able to reduce d-ribulose with reduced nicotinamide adenine dinucleotide phosphate (NADPH2) and d-xylulose with reduced nicotinamide adenine dinucleotide (NADH2). These extracts also oxidized d-arabitol with nicotinamide adenine dinucleotide phosphate and xylitol with nicotinamide adenine dinucleotide. No reduction of d-ribulose-5-phosphate or d-xylulose-5-phosphate was observed. An enzyme which reduced d-xylulose with NADH2 was purified 33-fold and characterized as a xylitol (→ d-xylulose) dehydrogenase. Similarly, an enzyme reducing d-ribulose with NADPH2 was purified 12-fold and characterized as a d-arabitol (→ d-ribulose) dehydrogenase. Alkaline and acid phosphatases were purified 50- and 40-fold, respectively, and their specificities were determined. Only the acid phosphatase had detectable activity on d-ribulose-5-phosphate. The data support the postulate that d-arabitol arises by dephosphorylation of d-ribulose-5-phosphate and reduction of d-ribulose by a NADPH2-linked d-arabitol (→ d-ribulose) dehydrogenase.

Full text

PDF
1186

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANDERSON R. L., WOOD W. A. Pathway of L-xylose and L-lyxose degradation in Aerobacter aerogenes. J Biol Chem. 1962 Feb;237:296–303. [PubMed] [Google Scholar]
  2. ANDERSON R. L., WOOD W. A. Purification and properties of L-xylulokinase. J Biol Chem. 1962 Apr;237:1029–1033. [PubMed] [Google Scholar]
  3. BALDWIN R. L., WOOD W. A., EMERY R. S. Conversion of lactate-C14 to propionate by the rumen microflora. J Bacteriol. 1962 Apr;83:907–913. doi: 10.1128/jb.83.4.907-913.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. BERNSTEIN I. A. Synthesis of ribose by the chick. J Biol Chem. 1953 Nov;205(1):317–329. [PubMed] [Google Scholar]
  5. BLAKLEY E. R., SPENCER J. F. Studies on the formation of D-arabitol by osmophilic yeasts. Can J Biochem Physiol. 1962 Dec;40:1737–1748. [PubMed] [Google Scholar]
  6. HICKMAN J., ASHWELL G. A sensitive and stereospecific enzymatic assay for xylulose. J Biol Chem. 1959 Apr;234(4):758–761. [PubMed] [Google Scholar]
  7. KRICHEVSKY M. I., WOOD W. A. Pathway of ribose 5-phosphate utilization in Microbacterium lacticum. J Bacteriol. 1961 Feb;81:246–257. doi: 10.1128/jb.81.2.246-257.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. MORTLOCK R. P., FOSSITT D. D., PETERING D. H., WOOD W. A. METABOLISM OF PENTOSES AND PENTITOLS BY AEROBACTER AEROGENES. 3. PHYSICAL AND IMMUNOLOGICAL PROPERTIES OF PENITOL DEHYDROGENASES AND PENTULOKINASES. J Bacteriol. 1965 Jan;89:129–135. doi: 10.1128/jb.89.1.129-135.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. PHARES E. F. Degradation of labeled propionic and acetic acids. Arch Biochem Biophys. 1951 Sep;33(2):173–178. doi: 10.1016/0003-9861(51)90094-x. [DOI] [PubMed] [Google Scholar]
  10. SCHMIDT G., BARTSCH G., LAUMONT M. C., HERMAN T., LISS M. Acid phosphatase of bakers' yeast: an enzyme of the external cell surface. Biochemistry. 1963 Jan-Feb;2:126–131. doi: 10.1021/bi00901a022. [DOI] [PubMed] [Google Scholar]
  11. SPENCER J. F., NEISH A. C., BLACKWOOD A. C., SALLANS H. R. Polyhydric alcohol production by osmophilic yeasts: studies with C14-labeled glucose. Can J Biochem Physiol. 1956 May;34(3):495–501. [PubMed] [Google Scholar]
  12. SPENCER J. F., SALLANS H. R. Production of polyhydric alcohols by osmophilic yeasts. Can J Microbiol. 1956 Apr;2(2):72–79. doi: 10.1139/m56-011. [DOI] [PubMed] [Google Scholar]
  13. SPENCER J. F., SHU P. Polyhydric alcohol production by osmophilic yeasts: effect of oxygen tension and inorganic phosphate concentration. Can J Microbiol. 1957 Jun;3(4):559–567. doi: 10.1139/m57-061. [DOI] [PubMed] [Google Scholar]
  14. SUOMALAINEN H., LINKO M., OURA E. Changes in the phosphatase activity of Baker's yeast during the growth phase and location of the phosphatases in the yeast cell. Biochim Biophys Acta. 1960 Jan 29;37:482–490. doi: 10.1016/0006-3002(60)90505-9. [DOI] [PubMed] [Google Scholar]
  15. TONINO G. J., STEYN-PARVE E. P. Localization of some phosphatases in yeast. Biochim Biophys Acta. 1963 Mar 12;67:453–469. doi: 10.1016/0006-3002(63)91851-1. [DOI] [PubMed] [Google Scholar]
  16. TORRIANI A. Influence of inorganic phosphate in the formation of phosphatases by Escherichia coli. Biochim Biophys Acta. 1960 Mar 11;38:460–469. doi: 10.1016/0006-3002(60)91281-6. [DOI] [PubMed] [Google Scholar]
  17. WEIMBERG R. Mode of formation of D-arabitol by Saccharomyces mellis. Biochem Biophys Res Commun. 1962 Aug 31;8:442–445. doi: 10.1016/0006-291x(62)90293-0. [DOI] [PubMed] [Google Scholar]
  18. WEIMBERG R., ORTON W. L. REPRESSIBLE ACID PHOSPHOMONOESTERASE AND CONSTITUTIVE PYROPHOSPHATASE OF SACCHAROMYCES MELLIS. J Bacteriol. 1963 Oct;86:805–813. doi: 10.1128/jb.86.4.805-813.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. WOOD W. A., GILFORD S. R. Recording of several simultaneous enzyme reactions facilitated by an automatic cuvette positioning attachment for the spectrophotometer. Anal Biochem. 1961 Dec;2:601–609. doi: 10.1016/0003-2697(61)90027-6. [DOI] [PubMed] [Google Scholar]
  20. WOOD W. A., McDONOUGH M. J., JACOBS L. B. Rihitol and D-arabitol utilization by Aerobacter aerogenes. J Biol Chem. 1961 Aug;236:2190–2195. [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES