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. 1965 May;89(5):1262–1270. doi: 10.1128/jb.89.5.1262-1270.1965

Degradation of Inorganic Polyphosphate in Mutants of Aerobacter aerogenes

F M Harold 1, Ruth L Harold 1
PMCID: PMC277638  PMID: 14292996

Abstract

Harold, F. M. (National Jewish Hospital, Denver, Colo.), and Ruth L. Harold. Degradation of inorganic polyphosphate in mutants of Aerobacter aerogenes. J. Bacteriol. 89:1262–1270. 1965.—Extracts of Aerobacter aerogenes contained two enzymes capable of degrading polyphosphate, polyphosphatase and polyphosphate kinase. By use of a suicide technique, a mutant (Pn-4) blocked in polyphosphate degradation was isolated; this mutant was found to lack polyphosphatase. The results indicate that polyphosphatase mediates the main pathway of polyphosphate degradation, and, therefore, that polyphosphate does not serve as a microbial phosphagen. A second mutant (Pn-3) exhibited transient accumulation of polyphosphate when cells were transferred to fresh growth medium. This strain was constitutive for elevated levels of polyphosphate kinase, polyphosphatase, and alkaline phosphatase; the transient accumulation of polyphosphate may be due to the shifting ratios of the biosynthetic and degradative enzymes during growth. These mutants were employed in studies on the competitive relationship between polyphosphate and nucleic acids. It was concluded that nucleic acid synthesis inhibits polyphosphate synthesis and also stimulates polyphosphate degradation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AMES B. N., MARTIN R. G. BIOCHEMICAL ASPECTS OF GENETICS: THE OPERON. Annu Rev Biochem. 1964;33:235–258. doi: 10.1146/annurev.bi.33.070164.001315. [DOI] [PubMed] [Google Scholar]
  2. DIRHEIMER G., EBEL J. P. [Demonstration of a polyphosphate-glucose-phosphotransferase in Corynebacterium xerosis]. C R Hebd Seances Acad Sci. 1962 Apr 9;254:2850–2852. [PubMed] [Google Scholar]
  3. HAROLD F. M. ACCUMULATION OF INORGANIC POLYPHOSPHATE IN AEROBACTER AEROGENES. I. RELATIONSHIP TO GROWTH AND NUCLEIC ACID SYNTHESIS. J Bacteriol. 1963 Aug;86:216–221. doi: 10.1128/jb.86.2.216-221.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. HAROLD F. M. ENZYMIC AND GENETIC CONTROL OF POLYPHOSPHATE ACCUMULATION IN AEROBACTER AEROGENES. J Gen Microbiol. 1964 Apr;35:81–90. doi: 10.1099/00221287-35-1-81. [DOI] [PubMed] [Google Scholar]
  5. HAROLD R. L., HAROLD F. M. Mutants of Aerobacter aerogenes blocked in the accumulation of inorganic polyphosphate. J Gen Microbiol. 1963 May;31:241–246. doi: 10.1099/00221287-31-2-241. [DOI] [PubMed] [Google Scholar]
  6. KORNBERG S. R. Adenosine triphosphate synthesis from polyphosphate by an enzyme from Escherichia coli. Biochim Biophys Acta. 1957 Nov;26(2):294–300. doi: 10.1016/0006-3002(57)90008-2. [DOI] [PubMed] [Google Scholar]
  7. LUBIN M. Enrichment of auxotrophic mutant populations by recycling. J Bacteriol. 1962 Mar;83:696–697. doi: 10.1128/jb.83.3.696-697.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LUBIN M. Selection of auxotrophic bacterial mutants by tritium-labeled thymidine. Science. 1959 Mar 27;129(3352):838–839. doi: 10.1126/science.129.3352.838. [DOI] [PubMed] [Google Scholar]
  9. MAAS W. K. STUDIES ON THE MECHANISM OF REPRESSION OF ARGININE BIOSYNTHESIS IN ESCHERICHIA COLI. II. DOMINANCE OF REPRESSIBILITY IN DIPLOIDS. J Mol Biol. 1964 Mar;8:365–370. doi: 10.1016/s0022-2836(64)80200-x. [DOI] [PubMed] [Google Scholar]
  10. MUHAMMED A., RODGERS A., HUGHES D. E. Purification and properties of a polymetaphosphatase from Corynebacterium xerosis. J Gen Microbiol. 1959 Jun;20(3):482–495. doi: 10.1099/00221287-20-3-482. [DOI] [PubMed] [Google Scholar]
  11. MUHAMMED A. Studies on biosynthesis of polymetaphosphate by an enzyme from Corynebacterium xerosis. Biochim Biophys Acta. 1961 Nov 25;54:121–132. doi: 10.1016/0006-3002(61)90945-3. [DOI] [PubMed] [Google Scholar]
  12. SMITH I. W., WILKINSON J. F., DUGUID J. P. Volutin production in Aerobacter aerogenes due to nutrient imbalance. J Bacteriol. 1954 Oct;68(4):450–463. doi: 10.1128/jb.68.4.450-463.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. SZYMONA M., OSTROWSKI W. INORGANIC POLYPHOSPHATE GLUCOKINASE OF MYCOBACTERIUM PHLEI. Biochim Biophys Acta. 1964 May 4;85:283–295. doi: 10.1016/0926-6569(64)90249-4. [DOI] [PubMed] [Google Scholar]
  14. TSUBOI K. K., PRICE T. D. Isolation, detection and measure of microgram quantities of labeled tissue nucleotides. Arch Biochem Biophys. 1959 Mar;81(1):223–237. doi: 10.1016/0003-9861(59)90192-4. [DOI] [PubMed] [Google Scholar]
  15. WILKINSON J. F., DUGUID J. P. The influence of cultural conditions on bacterial cytology. Int Rev Cytol. 1960;9:1–76. doi: 10.1016/s0074-7696(08)62744-8. [DOI] [PubMed] [Google Scholar]
  16. WINDER F. G., DENNENY J. M. The metabolism of inorganic polyphosphate in mycobacteria. J Gen Microbiol. 1957 Dec;17(3):573–585. doi: 10.1099/00221287-17-3-573. [DOI] [PubMed] [Google Scholar]

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