Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1965 May;89(5):1310–1313. doi: 10.1128/jb.89.5.1310-1313.1965

Differences in Cystine Dependence and Chromatographic Behavior Between Two Type 4 Echovirus Strains

Craig Wallis 1, Joseph L Melnick 1
PMCID: PMC277645  PMID: 14293003

Abstract

Wallis, Craig (Baylor University College of Medicine, Houston, Tex.), and Joseph L. Melnick. Differences in cystine dependence and chromatographic behavior between two type 4 echovirus strains. J. Bacteriol. 89:1310–1313. 1965.—Type 4 strains of echovirus, which are known to differ in the ease in which they can be neutralized, have been found to differ in other properties also. The prototype strain (Pesascek) differed from a readily neutralizable strain (DuToit) in that the Pesascek strain could be stabilized by cystine to thermal inactivation. Furthermore, the prototype virus required cyst(e)ine for growth under agar, whereas the DuToit strain was not affected. The viruses also reacted differently on anion-exchange resins. Pesascek virus had surface groups capable of exchanging for phosphate ions and, thus, was readily adsorbed, whereas DuToit virus was not bound by the resins under the same conditions. Only a small fraction of Pesascek virus was found to pass through the resin column. This fraction bred true, in that it yielded progeny that behaved like DuToit in not exchanging for phosphate. However, in neutralization tests, the progeny of this fraction retained the immunological property of the parent Pesascek virus.

Full text

PDF
1310

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARRON A. L., KARZON D. T. Characteristics of ECHO 4 (Shropshire) virus isolated during epidemic of aseptic meningitis. J Immunol. 1961 Nov;87:608–615. [PubMed] [Google Scholar]
  2. JAMISON R. M., MAYOR H. D., MELNICK J. L. Studies on ECHO 4 virus (Picornavirus group) and its intracellular development. Exp Mol Pathol. 1963 Apr;2:188–202. doi: 10.1016/0014-4800(63)90052-2. [DOI] [PubMed] [Google Scholar]
  3. LIEBHABER H., TAKEMOTO K. K. Alteration plaque morphology of EMC virus with polycations. Virology. 1961 Aug;14:502–504. doi: 10.1016/0042-6822(61)90349-x. [DOI] [PubMed] [Google Scholar]
  4. MALHERBE H., HARWIN R., SMITH A. H. An outbreak of aseptic meningitis associated with ECHO virus type 4. S Afr Med J. 1957 Dec 14;31(50):1261–1264. [PubMed] [Google Scholar]
  5. POHJANPELTO P. Stabilization of poliovirus by cystine. Virology. 1958 Oct;6(2):472–487. doi: 10.1016/0042-6822(58)90095-3. [DOI] [PubMed] [Google Scholar]
  6. TYTELL A. A., TOROP H. A., McCARTHY F. J. Plaque formation by myxovirus parainfluenza type 2 in grivet monkey kidney cells. Proc Soc Exp Biol Med. 1961 Dec;108:723–725. doi: 10.3181/00379727-108-27046. [DOI] [PubMed] [Google Scholar]
  7. WALLIS C., MELNICK J. L., BIANCHI M. Factors influencing enterovirus and reovirus growth and plaque formation. Tex Rep Biol Med. 1962;20:693–702. [PubMed] [Google Scholar]
  8. WALLIS C., MELNICK J. L. PHOTODYNAMIC INACTIVATION OF ENTEROVIRUSES. J Bacteriol. 1965 Jan;89:41–46. doi: 10.1128/jb.89.1.41-46.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. WALLIS C., MELNICK J. L. Suppression of adventitious agents in monkey kidney cultures. Tex Rep Biol Med. 1962;20:465–475. [PubMed] [Google Scholar]
  10. WALLIS C., MELNICK J. L. THERMOSENSITIVITY OF POLIOVIRUS. J Bacteriol. 1963 Sep;86:499–504. doi: 10.1128/jb.86.3.499-504.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. YOHN D. S., HAMMON W. M. ECHO-4 viruses: improved methods and strain selection for identification and serodiagnosis. Proc Soc Exp Biol Med. 1960 Oct;105:55–60. doi: 10.3181/00379727-105-26007. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES