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. 1965 May;89(5):1393–1401. doi: 10.1128/jb.89.5.1393-1401.1965

Automatic Classification of Staphylococci by Principal-Component Analysis and a Gradient Method1

L R Hill 1,2, L G Silvestri 1,2, P Ihm 1,2, G Farchi 1,2, P Lanciani 1,2
PMCID: PMC277657  PMID: 14293013

Abstract

Hill, L. R. (Università Statale, Milano, Italy), L. G. Silvestri, P. Ihm, G. Farchi, and P. Lanciani. Automatic classification of staphylococci by principal-component analysis and a gradient method. J. Bacteriol. 89:1393–1401. 1965.—Forty-nine strains from the species Staphylococcus aureus, S. saprophyticus, S. lactis, S. afermentans, and S. roseus were submitted to different taxometric analyses; clustering was performed by single linkage, by the unweighted pair group method, and by principal-component analysis followed by a gradient method. Results were substantially the same with all methods. All S. aureus clustered together, sharply separated from S. roseus and S. afermentans; S. lactis and S. saprophyticus fell between, with the latter nearer to S. aureus. The main purpose of this study was to introduce a new taxometric technique, based on principal-component analysis followed by a gradient method, and to compare it with some other methods in current use. Advantages of the new method are complete automation and therefore greater objectivity, execution of the clustering in a space of reduced dimensions in which different characters have different weights, easy recognition of taxonomically important characters, and opportunity for representing clusters in three-dimensional models; the principal disadvantage is the need for large computer facilities.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BAIRD-PARKER A. C. A classification of micrococci and staphylococci based on physiological and biochemical tests. J Gen Microbiol. 1963 Mar;30:409–427. doi: 10.1099/00221287-30-3-409. [DOI] [PubMed] [Google Scholar]
  2. BEERS R. J., LOCKHART W. R. Experimental methods in computer taxonomy. J Gen Microbiol. 1962 Sep;28:633–640. doi: 10.1099/00221287-28-4-633. [DOI] [PubMed] [Google Scholar]
  3. DEFAYOLLE M., COLOBERT L. [The species Streptococous faecalis. II. Study of homogeneity by means of factor analysis]. Ann Inst Pasteur (Paris) 1962 Oct;103:505–522. [PubMed] [Google Scholar]
  4. HILL L. R. The Adansonian classification of the staphylococci. J Gen Microbiol. 1959 Apr;20(2):277–283. doi: 10.1099/00221287-20-2-277. [DOI] [PubMed] [Google Scholar]
  5. LYSENKO O., SNEATH P. H. The use of models in bacterial classification. J Gen Microbiol. 1959 Apr;20(2):284–290. doi: 10.1099/00221287-20-2-284. [DOI] [PubMed] [Google Scholar]
  6. Rogers D. J., Tanimoto T. T. A Computer Program for Classifying Plants. Science. 1960 Oct 21;132(3434):1115–1118. doi: 10.1126/science.132.3434.1115. [DOI] [PubMed] [Google Scholar]
  7. SHAW C., STITT J. M., COWAN S. T. Staphylococci and their classification. J Gen Microbiol. 1951 Nov;5(5 Suppl):1010–1023. doi: 10.1099/00221287-5-5-1010. [DOI] [PubMed] [Google Scholar]
  8. SNEATH P. H. Some thoughts on bacterial classification. J Gen Microbiol. 1957 Aug;17(1):184–200. doi: 10.1099/00221287-17-1-184. [DOI] [PubMed] [Google Scholar]
  9. SNEATH P. H. The application of computers to taxonomy. J Gen Microbiol. 1957 Aug;17(1):201–226. doi: 10.1099/00221287-17-1-201. [DOI] [PubMed] [Google Scholar]

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