Abstract
Holt, Stanley C. (University of California, Davis), and Allen G. Marr. Isolation and purification of the intracytoplasmic membranes of Rhodospirillum rubrum. J. Bacteriol. 89:1413–1420. 1965.—When Rhodospirillum rubrum was disrupted in a French pressure cell, two colored bands were observed after density-gradient centrifugation: an upper band containing chromatophores and a lower band containing cell envelopes. A similar analysis of the pigmented components after disruption by osmotic shock revealed the presence of a third band intermediate between the upper and lower bands. Brief sonic treatment of the contents of this intermediate band produced typical chromatophores. The results establish that chromatophores are produced by fragmentation of the intracytoplasmic membrane.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BOATMAN E. S. OBSERVATIONS ON THE FINE STRUCTURE OF SPHEROPLASTS OF RHODOSPIRILLUM RUBRUM. J Cell Biol. 1964 Feb;20:297–311. doi: 10.1083/jcb.20.2.297. [DOI] [PMC free article] [PubMed] [Google Scholar]
- COHEN-BAZIRE G., SISTROM W. R., STANIER R. Y. Kinetic studies of pigment synthesis by non-sulfur purple bacteria. J Cell Physiol. 1957 Feb;49(1):25–68. doi: 10.1002/jcp.1030490104. [DOI] [PubMed] [Google Scholar]
- Cohen-Bazire G., Kunisawa R. SOME OBSERVATIONS ON THE SYNTHESIS AND FUNCTION OF THE PHOTOSYNTHETIC APPARATUS IN RHODOSPIRILLUM RUBRUM. Proc Natl Acad Sci U S A. 1960 Dec;46(12):1543–1553. doi: 10.1073/pnas.46.12.1543. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HOLT S. C., MARR A. G. LOCATION OF CHLOROPHYLL IN RHODOSPIRILLUM RUBRUM. J Bacteriol. 1965 May;89:1402–1412. doi: 10.1128/jb.89.5.1402-1412.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- NEWTON J. W., NEWTON G. A. Composition of the photoactive subcellular particles from Chromatium. Arch Biochem Biophys. 1957 Sep;71(1):250–265. doi: 10.1016/0003-9861(57)90026-7. [DOI] [PubMed] [Google Scholar]
- Park R. B., Biggins J. Quantasome: Size and Composition. Science. 1964 May 22;144(3621):1009–1011. doi: 10.1126/science.144.3621.1009. [DOI] [PubMed] [Google Scholar]
- ROBRISH S. A., MARR A. G. Location of enzymes in Azotobacteragilis. J Bacteriol. 1962 Jan;83:158–168. doi: 10.1128/jb.83.1.158-168.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- STOECKENIUS W. Some observations on negatively stained mitochondria. J Cell Biol. 1963 May;17:443–454. doi: 10.1083/jcb.17.2.443. [DOI] [PMC free article] [PubMed] [Google Scholar]