Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1962 Feb;83(2):284–296. doi: 10.1128/jb.83.2.284-296.1962

SEROLOGY OF THE SOLUBLE ANTIGENS OF CLOSTRIDIUM PERFRINGENS TYPES A-F BY AGAR-GEL DIFFUSION

Paul D Ellner 1, Carolyn D Bohan 1
PMCID: PMC277727  PMID: 13890020

Abstract

Ellner, Paul D. (University of Vermont, Burlington) and Carolyn D. Bohan. Serology of the soluble antigens of Clostridium perfringens types A–F by agar-gel diffusion. J. Bacteriol. 83:284–296. 1962.—A serological study by agar-gel diffusion of the soluble antigens of 39 strains of the Clostridium perfringens group has shown them to be extremely heterogeneous. Strain variation occurred within the six types, and common antigens shared among the six types were frequently observed. Attempts to produce type-specific sera by absorption were unsuccessful, due to incomplete removal of common antibodies.

Full text

PDF
284

Images in this article

288Image
on p.288
289Image
on p.289
290Image
on p.290
291Image
on p.291
292Image
on p.292
293Image
on p.293
294Image
on p.294

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BERLIN B. S., McKINNEY R. W. A simple device for making emulsified vaccines. J Lab Clin Med. 1958 Oct;52(4):657–658. [PubMed] [Google Scholar]
  2. BJORKLUND B., BERENGO A. Studies on the antigenic composition of some clostridia toxins with the aid of gel diffusion. Acta Pathol Microbiol Scand. 1954;34(1):79–86. doi: 10.1111/j.1699-0463.1954.tb00803.x. [DOI] [PubMed] [Google Scholar]
  3. ELEK S. D. The serological analysis of mixed flocculating systems by means of diffusion gradients. Br J Exp Pathol. 1949 Oct;30(5):484–500. [PMC free article] [PubMed] [Google Scholar]
  4. ELLNER P. D. A medium promoting rapid quantitative sporulation in Clostridium perfringens. J Bacteriol. 1956 Apr;71(4):495–496. doi: 10.1128/jb.71.4.495-496.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. FULTHORPE A. J., THOMSON R. O. Antigenic efficiency of tetanus toxoids modified by excess formalin or by heat and phenol. Immunology. 1960 Apr;3:126–134. [PMC free article] [PubMed] [Google Scholar]
  6. OAKLEY C. L., FULTHORPE A. J. Antigenic analysis by diffusion. J Pathol Bacteriol. 1953 Jan;65(1):49–60. doi: 10.1002/path.1700650105. [DOI] [PubMed] [Google Scholar]
  7. OAKLEY C. L., WARRACK G. H. Routine typing of Clostridium welchii. J Hyg (Lond) 1953 Mar;51(1):102–107. doi: 10.1017/s0022172400015527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. ORLANS E. S., JONES V. E. Studies on some soluble antigens of Clostridium welchii types B, C and D. Immunology. 1958 Jul;1(3):268–290. [PMC free article] [PubMed] [Google Scholar]
  9. Orr J. H., Reed G. B. Serological Types of Clostridium perfringens. J Bacteriol. 1940 Sep;40(3):441–448. doi: 10.1128/jb.40.3.441-448.1940. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. REED F. C. Lighting technic for the photography of agar diffusion plates. Tech Bull Regist Med Technol. 1960 Mar;30:48–50. [PubMed] [Google Scholar]
  11. Svec M. H., McCoy E. A Chemical and Immunological Study of the Capsular Polysaccharide of Clostridium perfringens. J Bacteriol. 1944 Jul;48(1):31–44. doi: 10.1128/jb.48.1.31-44.1944. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. WILLIS A. T., HOBBS G. Some new media for the isolation and identification of Clostridia. J Pathol Bacteriol. 1959 Apr;77(2):511–521. doi: 10.1002/path.1700770223. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES