Abstract
McDuff, C. R. (University of Wisconsin, Madison), Lois M. Jones, and J. B. Wilson. Characteristics of brucellaphage. J. Bacteriol. 83:324–329. 1962.—Methods of characterizing phage have been applied to a brucellaphage of Russian origin grown on its propagating strain, Brucella abortus R 19. Phage can be propagated by single plaque transfer. Phage titers of about 1010 particles per ml can be obtained by propagation on a young culture of R 19 in Albimi broth on a shaker at 37 C. After lyophilization, phage retains its activity during storage for at least 20 months at 4 C. Phage is stable in broth at pH values from 6 to 8 for 24 hr at 37 C. Some loss in activity results from heating for 1 hr at 60 C. All activity is lost in the presence of 10% chloroform. It has a slow adsorption rate (K = 3.6 × 10−11 ml/min), a latent period of 100 min, and a burst size of 121 particles. Electron micrographs indicate that the phage is approximately 65 mμ in diameter, polygonal in shape, with a short tail.
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- CARRERE L., ROUX J., MANDIN J. Obtention de souches lysogènes de Corynebacterium parvum et de Brucella melitensis. C R Seances Soc Biol Fil. 1956;150(8-9):1599–1600. [PubMed] [Google Scholar]
- JONES L. M. Comparison of phage typing with standard methods of species differentiation in brucellae. Bull World Health Organ. 1960;23:130–133. [PMC free article] [PubMed] [Google Scholar]
- KAESBERG P., SETO J. T., WILSON J. B. Electron microscopy and serology of staphylococcus phages. J Bacteriol. 1956 Dec;72(6):847–850. doi: 10.1128/jb.72.6.847-850.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KESSEL R. W., ARONSON M., BRAUN W. Effects of brucellaphage on extracellular and intracellular Brucella abortus. Proc Soc Exp Biol Med. 1960 Dec;105:447–450. doi: 10.3181/00379727-105-26136. [DOI] [PubMed] [Google Scholar]
- PARNAS J., FELTYNOWSKI A., BULIKOWSKI W. Anti-brucella phage. Nature. 1958 Dec 6;182(4649):1610–1611. doi: 10.1038/1821610a0. [DOI] [PubMed] [Google Scholar]
- Stinebring W. R., Braun W. BRUCELLAPHAGE. J Bacteriol. 1959 Nov;78(5):736–737. doi: 10.1128/jb.78.5.736-737.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WAHL R., BLUM-EMERIQUE L. Les bacteries semi-résistantes au bactériophage I. Techniques; souches et phage utilisés. lyse sur milieu gélosé. Ann Inst Pasteur (Paris) 1952 Jan;82(1):29–43. [PubMed] [Google Scholar]
- WILLIAMS R. C., FRASER D. Morphology of the seven T-bacteriophages. J Bacteriol. 1953 Oct;66(4):458–464. doi: 10.1128/jb.66.4.458-464.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
- van DRIMMELEN G. Bacteriophage typing applied to strains of Brucella organisms. Nature. 1959 Oct 3;184(Suppl 14):1079–1079. doi: 10.1038/1841079a0. [DOI] [PubMed] [Google Scholar]