Abstract
Kinsky, Stephen C. (Washington University, St. Louis, Mo.). Effect of polyene antibiotics on protoplasts of Neurospora crassa. J. Bacteriol. 83:351–358. 1962.—The polyene antibiotics nystatin, amphotericin B, and filipin induced shrinkage of Neurospora crassa protoplasts stabilized in sucrose. At low concentrations of filipin (5 μg per ml), or higher concentrations of nystatin and amphotericin B (20 μg per ml), shrinkage was followed by swelling and lysis (bursting) of protoplasts. The effect of various parameters, such as metabolic inhibitors, protoplast concentration, and polyene concentration, was determined. The available evidence is consistent with the hypothesis that polyene antibiotics act by altering cellular permeability, possibly by reaction with the cell membrane, of sensitive organisms.
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Selected References
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- BACHMANN B. J., BONNER D. M. Protoplasts from Neurospora crassa. J Bacteriol. 1959 Oct;78:550–556. doi: 10.1128/jb.78.4.550-556.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KINSKY S. C. Alterations in the permeability of Neurospora crassa due to polyene antibiotics. J Bacteriol. 1961 Dec;82:889–897. doi: 10.1128/jb.82.6.889-897.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KINSKY S. C. The effect of polyene antibiotics on permeability in Neurospora crassa. Biochem Biophys Res Commun. 1961 Apr 7;4:353–357. doi: 10.1016/0006-291x(61)90217-0. [DOI] [PubMed] [Google Scholar]
- LAMPEN J. O., ARNOW P. Inhibition of algae by nystatin. J Bacteriol. 1961 Aug;82:247–251. doi: 10.1128/jb.82.2.247-251.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MARINI F., ARNOW P., LAMPEN J. O. The effect of monovalent cations on the inhibition of yeast metabolism by nystatin. J Gen Microbiol. 1961 Jan;24:51–62. doi: 10.1099/00221287-24-1-51. [DOI] [PubMed] [Google Scholar]