Abstract
Doctor, V. M. (University of Texas, Houston). Microbiological assay for thymine using a mutant of Streptococcus faecalis. J. Bacteriol. 84:17–22. 1962.—A rapid microbiological assay procedure for the determination of thymine was devised. The test organism, Streptococcus faecalis Akt, a mutant of S. faecalis ATCC 8043, gives one-half the maximal growth response to 1.6 μg of thymine (assay range 0.5 to 2.5 μg) or to a molar equivalent (3.2 μg) of thymidine. Under these experimental conditions, 80 μg of 5-methylcytosine or 150 μg of thymidylic acid replace the thymine or thymidine requirement of the test organism. Addition of a mixture of adenine, guanine, and xanthine at a level of 40 μg/ml of each or higher to the basal medium, or addition of a mixture of deoxyadenosine and deoxyguanosine at a level of 80 μg/ml of each or higher to the basal medium, inhibited the thymine or thymidine growth response of the organism. Thymine activities of rat and chick tissues were determined by this procedure. The data on the recovery of added thymine to tissues gave 84 to 103% recovery. Thymus, spleen, small intestine, and ventral prostate were higher in thymine activities as compared with other tissues.
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- ELSON D., CHARGAFF E. On the desoxyribonucleic acid content of sea urchin gametes. Experientia. 1952 Apr 15;8(4):143–145. doi: 10.1007/BF02170221. [DOI] [PubMed] [Google Scholar]
- HOLMES W. L., WELCH A. D. A rapid padplate microbiologic assay for thymine. Proc Soc Exp Biol Med. 1954 Jul;86(3):564–566. [PubMed] [Google Scholar]
- LANSFORD E. M., Jr, HARDING W. M., SHIVE W. Thymidine determination by some microbiological assay methods. Arch Biochem Biophys. 1958 Jan;73(1):180–190. doi: 10.1016/0003-9861(58)90253-4. [DOI] [PubMed] [Google Scholar]
- ROTHERHAM J., SCHNEIDER W. C. Deoxyribosyl compounds in animal tissues. J Biol Chem. 1958 Jun;232(2):853–858. [PubMed] [Google Scholar]
- Stokes J. L. Substitution of Thymine for "Folic Acid" in the Nutrition of Lactic Acid Bacteria. J Bacteriol. 1944 Aug;48(2):201–209. doi: 10.1128/jb.48.2.201-209.1944. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ZAMENHOF S., GRIBIFF G. E. coli containing 5-bromouracil in its deoxyribonucleic acid. Nature. 1954 Aug 14;174(4424):307–308. doi: 10.1038/174307a0. [DOI] [PubMed] [Google Scholar]