Abstract
Tsuji, Kiyoshi (National Canners Association Research Laboratory, Berkeley, Calif.) and William E. Perkins. Sporulation of Clostridium botulinum I. Selection of an aparticulate sporulation medium. J. Bacteriol. 84:81–85. 1962.—The thermal resistances of Clostridium botulinum spores produced in seven different liquid media were compared. A 5% solution of a commercially produced mixture of dehydrated enzymatic hydrolyzates of casein and animal tissues yielded spores of maximal thermostability. Sporulation in this medium was almost complete under the conditions employed. The suspensions were essentially free of vegetative cells and sporangia and could be thoroughly washed. The incubation time at 30 C was found to have a negligible effect on thermal resistance for periods between 4 and 21 days. Supplementing the medium yielding the most thermolabile spores with various divalent cations did not enhance the thermal resistance of the spores produced. The identical amino acids and related compounds were found in spores exhibiting the maximal and minimal thermal resistance when they were analyzed chromatographically. The more thermostable spores contained a higher concentration of glucosamine.
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- AMAHA M., ORDAL Z. J. Effect of divalent cations in the sporulation medium on the thermal death rate of Bacillus coagulans var. thermoacidurans. J Bacteriol. 1957 Nov;74(5):596–604. doi: 10.1128/jb.74.5.596-604.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ANDERSEN A. A. A rapid plate method of counting spores of Clostridium botulinum. J Bacteriol. 1951 Oct;62(4):425–432. doi: 10.1128/jb.62.4.425-432.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Halvorson H. O., Ziegler N. R. Application of Statistics to Problems in Bacteriology: I. A Means of Determining Bacterial Population by the Dilution Method. J Bacteriol. 1933 Feb;25(2):101–121. doi: 10.1128/jb.25.2.101-121.1933. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PIEZ K. A., MORRIS L. A modified procedure for the automatic analysis of amino acids. Anal Biochem. 1960 Nov;1:187–201. doi: 10.1016/0003-2697(60)90045-2. [DOI] [PubMed] [Google Scholar]
- SLEPECKY R., FOSTER J. W. Alterations in metal content of spores of Bacillus megaterium and the effect on some spore properties. J Bacteriol. 1959 Jul;78(1):117–123. doi: 10.1128/jb.78.1.117-123.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SUGIYAMA H. Studies on factors affecting the heat resistance of spores of Clostridium botulinum. J Bacteriol. 1951 Jul;62(1):81–96. doi: 10.1128/jb.62.1.81-96.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]