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. 1969 Feb;62(2):415–419. doi: 10.1073/pnas.62.2.415

A SPECIFIC ANTI-PROINSULIN SERUM AND THE PRESENCE OF PROINSULIN IN CALF SERUM*

C C Yip 1, J Logothetopoulos 1
PMCID: PMC277814  PMID: 5256220

Abstract

A specific antibody against proinsulin has been obtained by adsorbing the original anti-proinsulin guinea pig serum with a solid immunosorbent of Sephadex-insulin. The specificity of the antibody against antigenic determinants of proinsulin was established by radioimmunoassay and by passive cutaneous anaphylaxis (PCA) tests. The presence of proinsulin in calf serum has been demonstrated by (a) gel filtration of an acid alcohol extract of the serum followed by immunoassays of the fractions using anti-insulin or the specific anti-proinsulin serum, and (b) direct assay of calf serum with the specific anti-proinsulin serum.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Axén R., Porath J. Chemical coupling of enzymes to cross-linked dextran ('Sephadex'). Nature. 1966 Apr 23;210(5034):367–369. doi: 10.1038/210367a0. [DOI] [PubMed] [Google Scholar]
  2. Chance R. E., Ellis R. M., Bromer W. W. Porcine proinsulin: characterization and amino acid sequence. Science. 1968 Jul 12;161(3837):165–167. doi: 10.1126/science.161.3837.165. [DOI] [PubMed] [Google Scholar]
  3. HUNTER W. M., GREENWOOD F. C. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962 May 5;194:495–496. doi: 10.1038/194495a0. [DOI] [PubMed] [Google Scholar]
  4. Roth J., Gorden P., Pastan I. "Big insulin": a new component of plasma insulin detected by immunoassay. Proc Natl Acad Sci U S A. 1968 Sep;61(1):138–145. doi: 10.1073/pnas.61.1.138. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Rubenstein A. H., Cho S., Steiner D. F. Evidence for proinsulin in human urine and serum. Lancet. 1968 Jun 22;1(7556):1353–1355. doi: 10.1016/s0140-6736(68)92040-0. [DOI] [PubMed] [Google Scholar]
  6. Steiner D. F., Cunningham D., Spigelman L., Aten B. Insulin biosynthesis: evidence for a precursor. Science. 1967 Aug 11;157(3789):697–700. doi: 10.1126/science.157.3789.697. [DOI] [PubMed] [Google Scholar]
  7. Steiner D. F., Oyer P. E. The biosynthesis of insulin and a probable precursor of insulin by a human islet cell adenoma. Proc Natl Acad Sci U S A. 1967 Feb;57(2):473–480. doi: 10.1073/pnas.57.2.473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Yip C. C., Lin B. J. Amino acid composition of bovine 'proinsulin'. Biochem Biophys Res Commun. 1967 Nov 17;29(3):382–387. doi: 10.1016/0006-291x(67)90467-6. [DOI] [PubMed] [Google Scholar]
  9. Yip C. C. Terminal amino group determination, peptide mapping, biological and immunological activity of an insulin-like protein and the amino acid compositions of its two S-sulfo-polypeptide chains. Arch Biochem Biophys. 1968 Sep 20;127(1):741–748. doi: 10.1016/0003-9861(68)90284-1. [DOI] [PubMed] [Google Scholar]

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