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. 1969 Feb;62(2):558–565. doi: 10.1073/pnas.62.2.558

THE ROLES OF SYNTHESIS AND DEGRADATION IN DETERMINING TISSUE CONCENTRATIONS OF LACTATE DEHYDROGENASE-5*

Paul J Fritz 1,2,†, Elliot S Vesell 1,2,‡, E Lucile White 1,2,†, Kenneth M Pruitt 1,2,†
PMCID: PMC277841  PMID: 5256233

Abstract

C14-labeled amino acids were incorporated in vivo into LDH-5 of rat tissues. Different amounts of LDH-5 in liver, heart muscle, and skeletal muscle resulted from differences in rates of both intracellular degradation and synthesis. Rate constants for LDH-5 synthesis were 65, 2, and 5 picomoles per day per gram in rat liver, heart muscle, and skeletal muscle, respectively; rate constants for degradation were 0.041, 0.399, and 0.018 reciprocal days, respectively. The corresponding half-lives for LDH-5 in these tissues were 16, 1.6, and 31 days.

Markedly divergent rates of LDH-5 catabolism in various tissues suggest the possibility that one isozyme with a slow rate of destruction may serve to maintain critical enzymatic activity in a tissue where rapid degradation of another isozyme occurs.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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