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. 1969 Feb;62(2):581–588. doi: 10.1073/pnas.62.2.581

STEREOCHEMICAL ANALYSIS OF THE SPECIFICITY OF PANCREATIC RNASE WITH POLYFORMYCIN AS SUBSTRATE: DIFFERENTIATION OF THE TRANSPHOSPHORYLATION AND HYDROLYSIS REACTIONS*

D C Ward 1,2, W Fuller 1,2,†, E Reich 1,2
PMCID: PMC277847  PMID: 5256234

Abstract

A stereochemical analysis of the substrate and inhibitor specificities of bovine pancreatic ribonuclease A is presented. A scheme is proposed in which the binding specificity for this protein-nucleic acid interaction is rationalized in terms of a simple system of H-bonds. The functional groups that govern substrate binding for transphosphorylation and hydrolysis, respectively, are considered and differentiated, and predictions are offered concerning the interaction of presumptive substrates with RNase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DONOHUE J., TRUEBLOOD K. N. Base pairing in DNA. J Mol Biol. 1960 Dec;2:363–371. doi: 10.1016/s0022-2836(60)80047-2. [DOI] [PubMed] [Google Scholar]
  2. Emerson T. R., Swan R. J., Ulbricht T. L. Optical rotatory dispersion of nucleic acid derivatives. 8. The conformation of pyrimidine nucleosides in solution. Biochemistry. 1967 Mar;6(3):843–850. doi: 10.1021/bi00855a026. [DOI] [PubMed] [Google Scholar]
  3. Gassen H. G., Witzel H. Zum Mechanismus der Ribonuclease-Reaktion. 1. Die Aufgabe der Pyrimidinbase bei der Reaktion. Eur J Biochem. 1967 Mar;1(1):36–45. doi: 10.1111/j.1432-1033.1967.tb00041.x. [DOI] [PubMed] [Google Scholar]
  4. HUMMEL J. P., KALNITSKY G. MECHANISMS OF CERTAIN PHOSPHOTRANSFERASE REACTIONS: CORRELATION OF STRUCTURE AND CATALYSIS IN SOME SELECTED ENZYMES. Annu Rev Biochem. 1964;33:15–50. doi: 10.1146/annurev.bi.33.070164.000311. [DOI] [PubMed] [Google Scholar]
  5. HUMMEL J. P., VER PLOEG D. A., NELSON C. A. The interaction between ribonuclease and mononucleotides as measured spectrophotometrically. J Biol Chem. 1961 Dec;236:3168–3172. [PubMed] [Google Scholar]
  6. Haschemeyer A. E., Rich A. Nucleoside conformations: an analysis of steric barriers to rotation about the glycosidic bond. J Mol Biol. 1967 Jul 28;27(2):369–384. doi: 10.1016/0022-2836(67)90026-5. [DOI] [PubMed] [Google Scholar]
  7. LEVIN D. H. The polymerization of 8-azaguanosine 5-diphosphate by polynucleotide phosphorylase. Biochim Biophys Acta. 1962 Jul 9;61:75–81. doi: 10.1016/0926-6550(62)90032-4. [DOI] [PubMed] [Google Scholar]
  8. Massoulié J., Michelson A. M., Pochon F. Polynucleotide analogues. VI. Physical studies on 5-substituted pyrimidine polynucleotides. Biochim Biophys Acta. 1966 Jan 18;114(1):16–26. [PubMed] [Google Scholar]
  9. UKITA T., WAKU K., IRIE M., HOSHINO O. Research on pancreatic ribonuclease. I. The inhibition of cyclic phospho-diesterase activity of bovine pancreatic ribonuclease by several substrate analogues. J Biochem. 1961 Nov;50:405–415. doi: 10.1093/oxfordjournals.jbchem.a127467. [DOI] [PubMed] [Google Scholar]
  10. Ward D. C., Reich E. Conformational properties of polyformycin: a polyribonucleotide with individual residues in the syn conformation. Proc Natl Acad Sci U S A. 1968 Dec;61(4):1494–1501. doi: 10.1073/pnas.61.4.1494. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Wyckoff H. W., Hardman K. D., Allewell N. M., Inagami T., Johnson L. N., Richards F. M. The structure of ribonuclease-S at 3.5 A resolution. J Biol Chem. 1967 Sep 10;242(17):3984–3988. [PubMed] [Google Scholar]

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