Skip to main content
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1969 Feb;62(2):589–596. doi: 10.1073/pnas.62.2.589

VIRUS-SPECIFIC NUCLEIC ACIDS IN SV40-EXPOSED HAMSTER EMBRYO CELL LINES: CORRELATION WITH S AND T ANTIGENS*

Myron J Levin 1,2,3,, Michael N Oxman 1,2,3,, George Th Diamandopoulos 1,2,3,§, Arthur S Levine 1,2,3,, Patrick H Henry 1,2,3,, John F Enders 1,2,3,**
PMCID: PMC277849  PMID: 4307716

Abstract

A number of homologous SV40-exposed hamster embryonic cell lines were examined for the presence of RNA complementary to SV40 DNA. Only those lines containing the SV40 T antigen were found to have such virus-specific RNA. In lines containing the SV40 S antigen, but not the SV40 T antigen, virus-specific RNA was not detected. These findings suggest that the S antigen is not coded for directly by the SV40 genome.

Full text

PDF
589

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aloni Y., Winocour E., Sachs L. Characterization of the simian virus 40-specific RNA in virus-yielding and transformed cells. J Mol Biol. 1968 Feb 14;31(3):415–429. doi: 10.1016/0022-2836(68)90418-x. [DOI] [PubMed] [Google Scholar]
  2. Anderer F. A., Koch M. A., Schlumberger H. D. Structure of simian virus 40. 3. Alkaline degradation of the virus particle. Virology. 1968 Mar;34(3):452–458. doi: 10.1016/0042-6822(68)90065-2. [DOI] [PubMed] [Google Scholar]
  3. BLACK P. H., ROWE W. P. VIRAL STUDIES OF SV40 TUMORIGENESIS IN HAMSTERS. J Natl Cancer Inst. 1964 Jan;32:253–265. [PubMed] [Google Scholar]
  4. CRAWFORD L. V., BLACK P. H. THE NUCLEIC ACID OF SIMIAN VIRUS 40. Virology. 1964 Nov;24:388–392. doi: 10.1016/0042-6822(64)90176-x. [DOI] [PubMed] [Google Scholar]
  5. Diamandopoulos G. T., Tevethia S. S., Rapp F., Enders J. F. Development of S and T antigens and oncogenicity in hamster embryonic cell lines exposed to SV40. Virology. 1968 Feb;34(2):331–336. doi: 10.1016/0042-6822(68)90243-2. [DOI] [PubMed] [Google Scholar]
  6. EAGLE H. Amino acid metabolism in mammalian cell cultures. Science. 1959 Aug 21;130(3373):432–437. doi: 10.1126/science.130.3373.432. [DOI] [PubMed] [Google Scholar]
  7. Fujinaga K., Green M. The mechanism of viral carcinogenesis by DNA mammalian viruses: viral-specific RNA in polyribosomes of adenovirus tumor and transformed cells. Proc Natl Acad Sci U S A. 1966 Jun;55(6):1567–1574. doi: 10.1073/pnas.55.6.1567. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. GILDEN R. V., CARP R. I., TAGUCHI F., DEFEND V. THE NATURE AND LOCALIZATION OF THE SV 40-INDUCED COMPLEMENT-FIXING ANTIGEN. Proc Natl Acad Sci U S A. 1965 Mar;53:684–692. doi: 10.1073/pnas.53.3.684. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gerber P. Studies on the transfer of subviral infectivity from SV40-induced hamster tumor cells to indicator cells. Virology. 1966 Apr;28(4):501–509. doi: 10.1016/0042-6822(66)90234-0. [DOI] [PubMed] [Google Scholar]
  10. Gillespie D., Spiegelman S. A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J Mol Biol. 1965 Jul;12(3):829–842. doi: 10.1016/s0022-2836(65)80331-x. [DOI] [PubMed] [Google Scholar]
  11. HOPPS H. E., BERNHEIM B. C., NISALAK A., TJIO J. H., SMADEL J. E. BIOLOGIC CHARACTERISTICS OF A CONTINUOUS KIDNEY CELL LINE DERIVED FROM THE AFRICAN GREEN MONKEY. J Immunol. 1963 Sep;91:416–424. [PubMed] [Google Scholar]
  12. KIRBY K. S. ISOLATION AND CHARACTERIZATION OF RIBOSOMAL RIBONUCLEIC ACID. Biochem J. 1965 Jul;96:266–269. doi: 10.1042/bj0960266. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kluchareva T. E., Shachanina K. L., Belova S., Chibisova V., Deichman G. I. Use of immunofluorescence for detection of specific membrane antigen in simian virus 40-infected nontransformed cells. J Natl Cancer Inst. 1967 Nov;39(5):825–832. [PubMed] [Google Scholar]
  14. Oda K., Dulbecco R. Regulation of transcription of the SV40 DNA in productively infected and in transformed cells. Proc Natl Acad Sci U S A. 1968 Jun;60(2):525–532. doi: 10.1073/pnas.60.2.525. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Oxman M. N., Baron S., Black P. H., Takemoto K. K., Habel K., Rowe W. P. The effect of interferon on SV-40 T antigen production in SV-40-transformed cells. Virology. 1967 May;32(1):122–127. doi: 10.1016/0042-6822(67)90260-7. [DOI] [PubMed] [Google Scholar]
  16. POPE J. H., ROWE W. P. DETECTION OF SPECIFIC ANTIGEN IN SV40-TRANSFORMED CELLS BY IMMUNOFLUORESCENCE. J Exp Med. 1964 Aug 1;120:121–128. doi: 10.1084/jem.120.2.121. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. RAPP F., MELNICK J. L., KITAHARA T. TUMOR AND VIRUS ANTIGENS OF SIMIAN VIRUS 40: DIFFERENTIAL INHIBITION OF SYNTHESIS BY CYTOSINE ARABINOSIDE. Science. 1965 Feb 5;147(3658):625–627. doi: 10.1126/science.147.3658.625. [DOI] [PubMed] [Google Scholar]
  18. Rose J. A., Reich P. R., Weissman S. M. RNA production in adenovirus-infected KB cells. Virology. 1965 Dec;27(4):571–579. doi: 10.1016/0042-6822(65)90183-2. [DOI] [PubMed] [Google Scholar]
  19. Rowe W. P., Baum S. G., Pugh E., Hoggan M. D. Studies of adenovirus SV40 hybrid viruses. I. Assay system and further evidence for hybridization. J Exp Med. 1965 Nov 1;122(5):943–954. doi: 10.1084/jem.122.5.943. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. SCHERRER K., DARNELL J. E. Sedimentation characteristics of rapidly labelled RNA from HeLa cells. Biochem Biophys Res Commun. 1962 Jun 4;7:486–490. doi: 10.1016/0006-291x(62)90341-8. [DOI] [PubMed] [Google Scholar]
  21. TEVETHIA S. S., KATZ M., RAPP F. NEW SURFACE ANTIGEN IN CELLS TRANSFORMED BY SIMIAN PAPOVAVIRUS SV40. Proc Soc Exp Biol Med. 1965 Jul;119:896–901. doi: 10.3181/00379727-119-30330. [DOI] [PubMed] [Google Scholar]
  22. Tevethia S. S., Diamandopoulos G. T., Rapp F., Enders J. F. Lack of relationship between virus-specific surface and transplantation antigens in hamster cells transformed by simian papovavirus SV40. J Immunol. 1968 Dec;101(6):1192–1198. [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES