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. Author manuscript; available in PMC: 2010 Jun 16.
Published in final edited form as: Biochemistry. 2009 Jun 16;48(23):5131–5141. doi: 10.1021/bi900607q

Figure 5.

Figure 5

UV-visible absorbance spectra of the N537A (-537Ala-Gly-) variant during a reductive titration with sodium dithionite in 50 mM sodium phosphate buffer, pH 7.0 at 25 °C under anaerobic conditions. The spectral changes shown were also qualitatively similar to those obtained for the N537G (-537Gly-Gly-) variant. Inset: Representative Nernst plots for the determinations of two-electron midpoint potentials for the N537A (-537Ala-Gly-) (closed circles) and the N537G (-537Gly-Gly-) (open circles) variants.