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. Author manuscript; available in PMC: 2010 Jun 16.
Published in final edited form as: Biochemistry. 2009 Jun 16;48(23):5131–5141. doi: 10.1021/bi900607q

Figure 6.

Figure 6

Changes in the absorbance spectrum of FMN when bound to the apoprotein form of the -537Gly-Ala- variant. An FMN solution (~5 μM) in 50 mM sodium phosphate buffer pH 7.0 at 25 °C was titrated with sub-stoichiometric amounts of freshly prepared apoprotein. The main panel shows the absorbance of FMN before (solid line) and after (dashed line) the addition of saturating amounts of the apoprotein. Inset: Difference spectra obtained during the titration. All spectra are corrected for dilution and, if necessary, for some light scattering.