Figure 9.—
cdc17-1 significantly enhances the growth defect in ten1-ts, but not cdc13-1 strains. (A) Growth of single and double mutants. Ten-fold serial dilutions of strains were incubated on YPD at the indicated temperatures. cdc13-1 cdc17-1 strains were obtained by tetrad dissection. cdc17-1 ten1-ts strains were obtained by plasmid shuffle from parental strain hC1678, using plasmids pCN284, pCN309, pCN311, pCN358, and pCN359. The ten1-ts single mutant plates were incubated at the same time as the double mutants. (WT is hC160). (B) In-gel hybridization analysis of ssTG1-3 in G2/M arrested cells. Strains were synchronized in nocodazole at 23°, and then split and incubated 3 more hr in nocodazole at 23° or 36°. DNA was isolated under native conditions; equivalent DNA concentrations were digested with XhoI and analyzed by in-gel hybridization. The total DNA loaded in each lane is similar, as visualized by ethidium bromide staining, shown below denatured gel.