Abstract
Firshein, William (Wesleyan University, Middletown, Conn.). Effect of manganese and enzymatic deoxyribonucleic acid digests on population changes and respiration of pneumococci. J. Bacteriol. 84:478–484. 1962.—Deoxyribonucleic acid (DNA; Na salt) treated with deoxyribonuclease and Mn++ (as MnSO4) stimulated R (avirulent) to S (virulent) population changes in vitro. Under certain environmental conditions and with certain strains, Mn++ alone elicited these changes, but, in most cases, both digested DNA and Mn++ were necessary for maximal effect. These population changes were due to a selective stimulation of the multiplication of S cells and a delay in S-cell lysis, with either a slight inhibitory effect or no effect against the multiplication of R cells. The magnitude of the population change depended upon the presence of digested DNA, the level of Mn++, the strain, and the concentration of basal medium. Mn++ enhanced respiration of S cells while inhibiting that of R cells.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- FIRSHEIN W., BRAUN W. Effects of enzymatic deoxyribonucleic acid digests on population changes and deoxyribonucleic acid synthesis of pneumococci. J Bacteriol. 1960 Feb;79:246–260. doi: 10.1128/jb.79.2.246-260.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FIRSHEIN W. Effects of deoxyribonucleic acid products on deoxyribonucleic acid synthesis of virulent and avirulent pneumococci. J Bacteriol. 1961 Aug;82:169–180. doi: 10.1128/jb.82.2.169-180.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FIRSHEIN W. Effects of deoxyribonucleic acid products on respiration of virulent and avirulent pneumococci. J Bacteriol. 1961 Aug;82:181–186. doi: 10.1128/jb.82.2.181-186.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Firshein W., Braun W. ON THE NATURE OF THE SELECTIVE EFFECTS OF DEOXYRIBONUCLEIC ACID DIGESTS UPON PNEUMOCOCCI OF DIFFERENT VIRULENCE. Proc Natl Acad Sci U S A. 1958 Sep 15;44(9):918–923. doi: 10.1073/pnas.44.9.918. [DOI] [PMC free article] [PubMed] [Google Scholar]