Abstract
Quinto, Grace (University of Kentucky College of Medicine, Lexington). Nutrition of five Bacteroides strains. J. Bacteriol. 84:559–562. 1962.—Some of the nutritional requirements of five gram-negative anaerobic bacilli, including Ristella perfoetens, Zuberella clostridiformis, and three Bacteroides strains freshly isolated from clinical exudates, were investigated. A fluid maintenance medium was developed in which the three freshly isolated strains, C-4, C-7, and C-2795, grew maximally in 12 to 24 hr. The maintenance medium contained 2.0% Trypticase and Proteose Peptone, 0.5% glucose, and 0.1% sodium thioglycolate; it was adjusted to pH 7.2 and supplemented with 0.1 μg of hemin/ml. Strains C-4, C-7, and C-2795 were cultivated through 14 serial cultures in fluid maintenance medium containing 0.1 μg of hemin/ml. The most satisfactory inoculum was a 1:100 or 1:1,000 dilution of a 24-hr seed culture. All the strains except Z. clostridiformis grew serially in a defined medium. R. perfoetens required pantothenic acid, nicotinic acid, and the following amino acids: histidine, tryptophan, tyrosine, valine, phenylalanine, cystine, and probably arginine, glutamic acid, methionine, glycine, isoleucine, leucine, and lysine. The C-4, C-7, and C-2795 strains required the hemin supplement in defined medium, but not vitamins, purines, or pyrimidines.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Eggerth A. H., Gagnon B. H. The Bacteroides of Human Feces. J Bacteriol. 1933 Apr;25(4):389–413. doi: 10.1128/jb.25.4.389-413.1933. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GIBBONS R. J., MACDONALD J. B. Hemin and vitamin K compounds as required factors for the cultivation of certain strains of Bacteroides melaninogenicus. J Bacteriol. 1960 Aug;80:164–170. doi: 10.1128/jb.80.2.164-170.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GOODGAL S. H., HERRIOTT R. M. Studies on transformations of Hemophilus influenzae. I. Competence. J Gen Physiol. 1961 Jul;44:1201–1227. doi: 10.1085/jgp.44.6.1201. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Henthorne J. C., Thompson L., Beaver D. C. Gram-Negative Bacilli of the Genus Bacteroides. J Bacteriol. 1936 Mar;31(3):255–274. doi: 10.1128/jb.31.3.255-274.1936. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lewis K. H., Bedell M., Rettger L. F. Non-Sporulating Anaerobic Bacteria of the Intestinal Tract : II. Growth-Facilitating Factors. J Bacteriol. 1940 Aug;40(2):309–320. doi: 10.1128/jb.40.2.309-320.1940. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McVAY L. V., Jr, SPRUNT D. H. Bacteroides infections. Ann Intern Med. 1952 Jan;36(1):56–76. doi: 10.7326/0003-4819-36-1-56. [DOI] [PubMed] [Google Scholar]
- TAMIMI H. A., HILTBRAND W., LOERCHER H. Some growth requirements of Bacteroides fragilis. J Bacteriol. 1960 Oct;80:472–476. doi: 10.1128/jb.80.4.472-476.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]