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. 2009 Feb;58(2):186–195. doi: 10.1016/j.metabol.2008.09.012

Table 1.

Primer sequences and product sizes for qPCR

Gene Sequences forward and reverse primers (5′-3′) Product size (base pair)
apo B Forward AGAAAGAGAACAAAGCAGAGATTGTGG 170
Reverse CATGCTCCATTCTCACATGTTTA
MTP Forward AGT GAT TTG ATG TCC AAA ATG CT 160
Reverse AAC CAG AAA TGT CAA TGG CTA GA
mGPAT Forward CCA GAC ATT TTA CCA GGT TTG TC 177
Reverse CTG TCC TCA TCT TCT TCG TCA CT
HMG-CoA reductase Forward CAA AGA CAA TCC TGG AGA AAA TG 166
Reverse TGTTCCCTGCAGATCTTGTAA AT
ACAT-2 Forward ACATATGGAGGCTGTGAAGACAC 164
Reverse GGTGTAACTCTTGGGTCT TCC TT
GAPDH Forward GATGACATCAAGAAGGTGGTG A 206
Reverse ACCCTGTTGCTG TAG CCATATT