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. 1962 Nov;84(5):877–881. doi: 10.1128/jb.84.5.877-881.1962

SUBSTRATE SPECIFICITY OF A GLUCOSE PERMEASE OF ESCHERICHIA COLI1

Dexter Rogers a,2, Shon-Hua Yu a
PMCID: PMC277984  PMID: 13982383

Abstract

Rogers, Dexter (Utah State University, Logan) and Shon-hua Yu. Substrate specificity of a glucose permease of Escherichia coli. J. Bacteriol. 84:877–881. 1962.—A study was made of d-galactose uptake by galactose-negative Escherichia coli strain A (Weigle). Uptake probably occurred through a glucose-permease system, because d-glucose and a variety of nonmetabolizable glucose derivatives inhibited the accumulation of galactose and were themselves accumulated. d-Fructose did not inhibit galactose uptake. 6-Deoxy-d-galactose (d-fucose) was taken up by a different permease system. The glucose permease apparently favored pyranoses, and it required the 6-hydroxyl group of the substrate to a greater extent than any of the other hydroxyl groups. Although much of the absorbed glucose-permease substrate was recovered in the free form, a significant amount was recovered as the 6-phosphate ester. Depending on the conditions employed to study uptake, the 6-phosphate ester could amount to as much as 60% of the absorbed galactose.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BUTTIN G., COHEN G. N., MONOD J., RICKENBERG H. V. La galactoside-perméase d'Escherichia coli. Ann Inst Pasteur (Paris) 1956 Dec;91(6):829–857. [PubMed] [Google Scholar]
  2. COHEN G. N., MONOD J. Bacterial permeases. Bacteriol Rev. 1957 Sep;21(3):169–194. doi: 10.1128/br.21.3.169-194.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CRANE R. K. Intestinal absorption of sugars. Physiol Rev. 1960 Oct;40:789–825. doi: 10.1152/physrev.1960.40.4.789. [DOI] [PubMed] [Google Scholar]
  4. HORECKER B. L., THOMAS J., MONOD J. Galactose transport in Escherichia coli. I. General properties as studied in a galactokinaseless mutant. J Biol Chem. 1960 Jun;235:1580–1585. [PubMed] [Google Scholar]
  5. LEFEVRE P. G., MARSHALL J. K. Conformational specificity in a biological sugar transport system. Am J Physiol. 1958 Aug;194(2):333–337. doi: 10.1152/ajplegacy.1958.194.2.333. [DOI] [PubMed] [Google Scholar]
  6. MAGER J. Spermine as a protective agent against osmotic lysis. Nature. 1959 Jun 27;183:1827–1828. doi: 10.1038/1831827a0. [DOI] [PubMed] [Google Scholar]
  7. MATELES R. I. Ferricyanide reduction method for reducing sugars. Nature. 1960 Jul 16;187:241–242. doi: 10.1038/187241a0. [DOI] [PubMed] [Google Scholar]
  8. Morris D. L. Quantitative Determination of Carbohydrates With Dreywood's Anthrone Reagent. Science. 1948 Mar 5;107(2775):254–255. doi: 10.1126/science.107.2775.254. [DOI] [PubMed] [Google Scholar]
  9. PARDEE A. B. An inducible mechanism for accumulation of melibiose in Escherichia coli. J Bacteriol. 1957 Mar;73(3):376–385. doi: 10.1128/jb.73.3.376-385.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. ROGERS D., REITHEL F. J. Acid phosphatases of Escherichia coli. Arch Biochem Biophys. 1960 Jul;89:97–104. doi: 10.1016/0003-9861(60)90018-7. [DOI] [PubMed] [Google Scholar]
  11. ROTMAN B. Separate permeases for the accumulation of methyl-beta-D-galactoside and methyl-beta-D-thiogalactoside in Escherichia coli. Biochim Biophys Acta. 1959 Apr;32:599–601. doi: 10.1016/0006-3002(59)90659-6. [DOI] [PubMed] [Google Scholar]

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