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. 1962 Nov;84(5):1011–1015. doi: 10.1128/jb.84.5.1011-1015.1962

SIMPLIFIED ANTISTREPTOCOCCAL DEOXYRIBONUCLEASE ASSAY1

Irving Olitzky a, Sam Berkman a, Doris Bass a
PMCID: PMC278002  PMID: 13939966

Abstract

Olitzky, Irving (Bio-Science Laboratories, Los Angeles, Calif.), Sam Berkman, and Doris Bass. Simplified antistreptococcal deoxyribonuclease assay. J. Bacteriol. 84:1011–1015. 1962.—A simple antistreptococcal deoxyribonuclease assay has been devised in which dilutions of the patient's serum are reacted with deoxyribonuclease, and residual active enzyme is then detected by the Di Salvo technique using commercially available agar medium containing deoxyribonucleic acid. Use of this test as supplemental to antistreptolysin-O titrations leads to increased positive findings when searching for streptococcal antibodies.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DISALVO J. W. Desoxyribonuclease and coagulase activity of micrococci. Med Techn Bull. 1958 Sep-Oct;9(5):191–196. [PubMed] [Google Scholar]
  2. HARRIS T. N., HARRIS S. Turbidimetric measurement of streptococcal antihyaluronidase in the sera of patients with streptococcal infection and rheumatic fever. J Immunol. 1949 Nov;63(3):249–260. [PubMed] [Google Scholar]
  3. JEFFRIES C. D., HOLTMAN D. F., GUSE D. G. Rapid method for determining the activity of microorganisms on nucleic acids. J Bacteriol. 1957 Apr;73(4):590–591. doi: 10.1128/jb.73.4.590-591.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. McCARTY M. The inhibition of streptococcal desoxyribonuclease by rabbit and human antisera. J Exp Med. 1949 Dec;90(6):543–553. doi: 10.1084/jem.90.6.543. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. WANNAMAKER L. W., AYOUB E. M. Antibody titers in acute rheumatic fever. Circulation. 1960 Apr;21:598–614. doi: 10.1161/01.cir.21.4.598. [DOI] [PubMed] [Google Scholar]
  6. WANNAMAKER L. W. The differentiation of three distinct desoxyrlbonucleases of group A Streptococci. J Exp Med. 1958 Jun 1;107(6):797–812. doi: 10.1084/jem.107.6.797. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. WANNAMAKER L. W. The paradox of the antibody response to streptodornase: the usefulness of antidesoxyribonuclease B as an indication of streptococcal infection in patients with acute rheumatic fever. Am J Med. 1959 Oct;27:567–574. doi: 10.1016/0002-9343(59)90042-7. [DOI] [PubMed] [Google Scholar]

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