Abstract
Whang, H. Y. (Children's Hospital, Buffalo, N.Y.) and E. Neter. Immunological studies of a heterogenetic enterobacterial antigen (Kunin). J. Bacteriol. 84:1245–1250. 1962.—Study of the heterogenetic antigen revealed that it is present in relatively large amounts in supernatant fluids of heat-killed, agar-grown enterobacterial suspensions. The antigen modifies erythrocytes for agglutination by heterogenetic antibodies in Escherichia coli O14 antiserum. Of the highly purified lipopolysaccharides (10 μg/ml) tested, only a Shigella sonnei preparation had significant activity. In the hemagglutination-inhibition test, minimal activity was detected also in lipopoly-saccharides from E. coli O111 and various salmonellase. There was no parallelism in capacity of these lipopolysaccharides to neutralize heterogenetic and homologous O antibodies. The hemolytic modification of the hemagglutination test proved to be from two to eight times more sensitive than the hemagglutination method. Supernatant fluids of unheated agar-grown suspensions contained small amounts of antigen, and boiling of the bacterial suspensions resulted in the release of additional and substantial quantities. Heterogenetic antibodies did not cause agglutination of latex particles treated with heterogenetic antigen, but E. coli O14 antibodies agglutinated latex particles treated with the corresponding O antigen.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Anzai H., Neter E., Gorzynski E. A. DEMONSTRATION OF RANTZ ANTIGEN IN MEMBERS OF THE GENUS BACILLUS. J Bacteriol. 1960 Jul;80(1):142–143. doi: 10.1128/jb.80.1.142-143.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BOKKENHEUSER V., GORZYNSKI E. A., COHEN E., NETER E. Immune agglutination and lysis of antigen modified erythrocytes from various animal species. Proc Soc Exp Biol Med. 1962 May;110:94–98. doi: 10.3181/00379727-110-27435. [DOI] [PubMed] [Google Scholar]
- GORZYNSKI E. A., LUDERITZ O., NETER E., WESTPHAL O. The bacterial hemagglutination test for the demonstration of antibodies to Enterobacteriaceae. Ann N Y Acad Sci. 1956 Aug 10;66(1):141–156. doi: 10.1111/j.1749-6632.1956.tb40113.x. [DOI] [PubMed] [Google Scholar]
- LUDERITZ O., WESTPHAL O., SIEVERS K., KROGER E., NETER E., BRAUN O. H. Uber die Fixation von P32-markiertem Lipopolysaccharid (Endotoxin) aus Escherichia coli an menschlichen Erythrocyten. Biochem Z. 1958;330(1):34–46. [PubMed] [Google Scholar]
- NETER E., GORZYNSKI E. A. Studies on indirect staphylococcal hemagglutination. Z Immun exp ther. 1959 Oct-Nov;118:269–288. [PubMed] [Google Scholar]
- RANTZ L. A., RANDALL E., ZUCKERMAN A. Hemolysis and hemagglutination by normal and immune serums of erythrocytes treated with a nonspecies specific bacterial substance. J Infect Dis. 1956 Mar-Apr;98(2):211–222. doi: 10.1093/infdis/98.2.211. [DOI] [PubMed] [Google Scholar]