Abstract
Marr, Allen G. (University of California, Davis) and John L. Ingraham. Effect of temperature on composition of fatty acids in Escherichia coli. J. Bacteriol. 84:1260–1267. 1962.—Variations in the temperature of growth and in the composition of the medium alter the proportions of individual fatty acids in the lipids of Escherichia coli. As the temperature of growth is lowered, the proportion of unsaturated fatty acids (hexadecenoic and octadecenoic acids) increases. The increase in content of unsaturated acids with a decrease in temperature of growth occurs in both minimal and complex media. Cells harvested in the stationary phase contained large amounts of cyclopropane fatty acids (methylenehexadecanoic and methylene octadecanoic acids) in comparison with cells harvested during exponential growth. Cells grown in a chemostat, limited by the concentration of ammonium salts, show a much higher content of saturated fatty acids (principally palmitic acid) than do cells harvested from an exponentially-growing batch culture in the same medium. Cells grown in a chemostat, limited by the concentration of glucose, show a slightly higher content of unsaturated fatty acids than cells from the corresponding batch culture. The results do not indicate a direct relation between fatty acid composition and minimal growth temperature.
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Selected References
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- Dean H. K., Hilditch T. P. The body fats of the pig: The influence of body temperature on the composition of depot fats. Biochem J. 1933;27(6):1950–1956. doi: 10.1042/bj0271950. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fraenkel G., Hopf H. S. The physiological action of abnormally high temperatures on poikilothermic animals: Temperature adaptation and the degree of saturation of the phosphatides. Biochem J. 1940 Jul;34(7):1085–1092. doi: 10.1042/bj0341085. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KANESHIRO T., MARR A. G. cis-9,10-Methylene hexadecanoic acid from the phospholipids of Escherichia coli. J Biol Chem. 1961 Oct;236:2615–2619. [PubMed] [Google Scholar]
- MONOD J., COHEN-BAZIRE G., COHN M. Sur la biosynthèse de la beta-galactosidase (lactase) chez Escherichia coli; la spécificité de l'induction. Biochim Biophys Acta. 1951 Nov;7(4):585–599. doi: 10.1016/0006-3002(51)90072-8. [DOI] [PubMed] [Google Scholar]
- NG H., INGRAHAM J. L., MARR A. G. Damage and derepression in Escherichia coli resulting from growth at low temperatures. J Bacteriol. 1962 Aug;84:331–339. doi: 10.1128/jb.84.2.331-339.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]