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. 1963 Jan;85(1):168–176. doi: 10.1128/jb.85.1.168-176.1963

PROTOPLAST MEMBRANE OF STREPTOCOCCUS FAECALIS

Gerald D Shockman 1, Joseph J Kolb 1, Bohdan Bakay 1, Margaret J Conover 1, Gerrit Toennies 1
PMCID: PMC278104  PMID: 13988637

Abstract

Shockman, Gerald D. (Temple University School of Medicine, Philadelphia, Pa.), Joseph J. Kolb, Bohdan Bakay, Margaret J. Conover, and Gerrit Toennies. Protoplast membrane of Streptococcus faecalis. J. Bacteriol. 85:168–176. 1963.—The membrane fraction of Streptococcus faecalis (ATCC 9790) was isolated and purified, by a variety of procedures, from cultures that were grown under closely controlled conditions of physiological age and nutrition. The most satisfactory method required the use of lysozyme-to-cell ratios below 0.01 and the intermediate formation of protoplasts in osmotically protective media. Amino acid analyses of three of the membrane preparations indicated a characteristic and constant, but not unusual, pattern; 42% of the membranes from threonine-depleted and 49 to 55% of the membranes from log-phase cultures were accounted for as protein. Significant quantities of d-alanine or d-aspartic acid were not detected, indicating the absence of contaminating cell-wall substance. Essentially, all of the nitrogen was accounted for as amino acids. The lipid content of membranes from stationary-phase threonine-depleted (36%) and valine-depleted (40%) cultures was significantly higher than the corresponding fraction of exponential-phase cultures (28%). The phosphorus content of the membrane lipid was relatively constant (2.8 to 3.0%), and the nitrogen content was extremely low (0.12 to 0.26%). Thus, changes in the composition of the membrane fraction occurred during the transition of log-phase cells into threonine- or valine-depleted cells.

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Selected References

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  1. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. GILBY A. R., FEW A. V., McQUILLEN K. The chemical composition of the protoplast membrane of Micrococcus lysodeikticus. Biochim Biophys Acta. 1958 Jul;29(1):21–29. doi: 10.1016/0006-3002(58)90141-0. [DOI] [PubMed] [Google Scholar]
  3. GODSON G. N., HUNTER G. D., BUTLER J. A. Cellular components of Bacillus megaterium and their role in protein biosynthesis. Biochem J. 1961 Oct;81:59–68. doi: 10.1042/bj0810059. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. KOLB J. J., WEIDNER M. A., TOENNIES G. Microdetermination of lipid phosphorus as a measure of bacterial membrane substance. Anal Biochem. 1963 Jan;5:78–82. doi: 10.1016/0003-2697(63)90061-7. [DOI] [PubMed] [Google Scholar]
  5. MITCHELL P., MOYLE J. Autolytic release and osmotic properties of protoplasts from Staphylococcus aureus. J Gen Microbiol. 1957 Feb;16(1):184–194. doi: 10.1099/00221287-16-1-184. [DOI] [PubMed] [Google Scholar]
  6. MOORE S., STEIN W. H. A modified ninhydrin reagent for the photometric determination of amino acids and related compounds. J Biol Chem. 1954 Dec;211(2):907–913. [PubMed] [Google Scholar]
  7. McQuillen K., Roberts R. B., Britten R. J. SYNTHESIS OF NASCENT PROTEIN BY RIBOSOMES IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1959 Sep;45(9):1437–1447. doi: 10.1073/pnas.45.9.1437. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. SHOCKMAN G. D. Bacterial cell wall synthesis: the effect of threonine depletion. J Biol Chem. 1959 Sep;234:2340–2342. [PubMed] [Google Scholar]
  9. SHOCKMAN G. D., KOLB J. J., TOENNIES G. Relations between bacterial cell wall synthesis, growth phase, and autolysis. J Biol Chem. 1958 Feb;230(2):961–977. [PubMed] [Google Scholar]
  10. Shockman G. D., Conover M. J., Kolb J. J., Phillips P. M., Riley L. S., Toennies G. LYSIS OF STREPTOCOCCUS FAECALIS. J Bacteriol. 1961 Jan;81(1):36–43. doi: 10.1128/jb.81.1.36-43.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Shockman G. D., Conover M. J., Kolb J. J., Riley L. S., Toennies G. NUTRITIONAL REQUIREMENTS FOR BACTERIAL CELL WALL SYNTHESIS. J Bacteriol. 1961 Jan;81(1):44–50. doi: 10.1128/jb.81.1.44-50.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. TOENNIES G., BAKAY B., SHOCKMAN G. D. Bacterial composition and growth phase. J Biol Chem. 1959 Dec;234:3269–3275. [PubMed] [Google Scholar]
  13. TOENNIES G., SHOCKMAN G. D. Quantitative amino acid assimilation in homofermentative metabolism. Arch Biochem Biophys. 1953 Aug;45(2):447–458. doi: 10.1016/s0003-9861(53)80021-4. [DOI] [PubMed] [Google Scholar]
  14. WEIBULL C., BERGSTROM L. The chemical nature of the cytoplasmic membrane and cell wall of Bacillus megaterium, strain M. Biochim Biophys Acta. 1958 Nov;30(2):340–351. doi: 10.1016/0006-3002(58)90059-3. [DOI] [PubMed] [Google Scholar]
  15. WEIBULL C., ZACHARIAS B., BECKMAN H. Affinity of lyzyme to structural elements of the bacterial cell as studied with enzyme labelled with radioactive iodine. Nature. 1959 Nov 28;184(Suppl 22):1744–1745. doi: 10.1038/1841744a0. [DOI] [PubMed] [Google Scholar]

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