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. 1963 Jan;85(1):230–236. doi: 10.1128/jb.85.1.230-236.1963

STUDIES ON IMMUNITY IN ANTHRAX X.

Gel-Adsorbed Protective Antigen for Immunization of Man

Milton Puziss 1, George G Wright 1
PMCID: PMC278112  PMID: 13972632

Abstract

Puziss, Milton (Fort Detrick, Frederick, Md.) and George G. Wright. Studies on immunity in anthrax. X. Gel-adsorbed protective antigen for immunization of man. J. Bacteriol. 85:230–236. 1963.—Protective antigen in filtrates of anaerobic cultures of Bacillus anthracis was not readily precipitable by alum or other aluminum salts, but was adsorbed onto a preformed aluminum hydroxide gel under specific conditions. The adsorbed product was highly effective in immunizing rabbits, guinea pigs, and mice against challenge with virulent B. anthracis spores. Gel-adsorbed anaerobic antigens preserved with 1:10,000 thimerosal were unstable on storage at 4 C; replacement of this preservative with 1:40,000 benzethonium chloride produced a more stable product. Addition of 0.0009% formalin further increased the stability during accelerated aging at 37 C. The final product had high protective activity in animals and was well tolerated in man.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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