Abstract
Sutton, W. B. (The Lilly Research Laboratories, Indianapolis, Ind.). Relationship of the hexose monophosphate shunt to the endogenous metabolism of cell-free extracts of Mycobacterium phlei. J. Bacteriol. 85:476–484. 1963.—The endogenous reduction of 2,6-dichlorophenol-indophenol (DPIP) by cell-free extracts of Mycobacterium phlei has been linked to the presence of glucose-6-phosphate (G-6-P) dehydrogenase functioning in connection with a reduced triphosphopyridine nucleotide (TPN)-DPIP reductase. The necessary substrate and coenzyme, i.e., G-6-P and TPN, are contained in the cell-free bacterial extract. The only required addition to activate the system is a suitable electron acceptor. The accumulation of G-6-P and the presence of 6-phosphogluconic dehydrogenase in the cell-free extract suggest that the hexose monophosphate shunt mechanism is impaired by sonic treatment of M. phlei.
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Selected References
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- BANDURSKI R. S., AXELROD B. The chromatographic identification of some biologically important phosphate esters. J Biol Chem. 1951 Nov;193(1):405–410. [PubMed] [Google Scholar]
- BARTLETT G. R. Methods for the isolation of glycolytic intermediated by column chromatography with ion exchange resins. J Biol Chem. 1959 Mar;234(3):459–465. [PubMed] [Google Scholar]
- COLOWICK S. P., KAPLAN N. O., CIOTTI M. M. The reaction of pyridine nucleotide with cyanide and its analytical use. J Biol Chem. 1951 Aug;191(2):447–459. [PubMed] [Google Scholar]
- EDSON N. L. The intermediary metabolism of the mycobacteria. Bacteriol Rev. 1951 Sep;15(3):147–182. doi: 10.1128/br.15.3.147-182.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Edson N. L., Hunter G. J. Respiration and nutritional requirements of certain members of the genus Mycobacterium. Biochem J. 1943;37(5):563–571. doi: 10.1042/bj0370563. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GOLDMAN D. S. Enzyme systems in the mycobacteria. A review. Bibl Tuberc. 1961;16:1–44. [PubMed] [Google Scholar]
- HANES C. S., ISHERWOOD F. A. Separation of the phosphoric esters on the filter paper chromatogram. Nature. 1949 Dec 31;164(4183):1107-12, illust. doi: 10.1038/1641107a0. [DOI] [PubMed] [Google Scholar]
- LANGAN T. A., Jr, KAPLAN N. O., SHUSTER L. Formation of the nicotinic acid analogue of diphosphopyridine nucleotide after nicotinamide administration. J Biol Chem. 1959 Aug;234(8):2161–2168. [PubMed] [Google Scholar]
- NOGGLE G. R. Paper chromatography of some ketoheptoses. Arch Biochem Biophys. 1953 Mar;43(1):238–239. doi: 10.1016/0003-9861(53)90106-4. [DOI] [PubMed] [Google Scholar]
- SEBRELL W. H., Jr Some problems in food toxicology. Fed Proc. 1960 Sep;19(Suppl 4):31–32. [PubMed] [Google Scholar]
- SUTTON W. B. Isolation and properties of a lactic oxidative decarboxylase from Mycobacterium phlei. J Biol Chem. 1954 Sep;210(1):309–320. [PubMed] [Google Scholar]
- SUTTON W. B. Mechanism of action and crystallization of lactic oxidative decarboxylase from Mycobacterium phlei. J Biol Chem. 1957 May;226(1):395–405. [PubMed] [Google Scholar]
- SUTTON W. B. Sulfhydryl and prosthetic groups of lactic oxidative decarboxylase from Mycobacterium phlei. J Biol Chem. 1955 Oct;216(2):749–761. [PubMed] [Google Scholar]
- VOLK W. A., MYRVEK Q. N. Intermediary metabolism of glucose by mycobacterium smegmatis. Am Rev Tuberc. 1956 Apr;73(4):589–592. doi: 10.1164/artpd.1956.73.4.589. [DOI] [PubMed] [Google Scholar]
- WEBER M. M., SWARTZ M. N. Pyridine nucleotide coenzymes of Mycobacterium phlei. Arch Biochem Biophys. 1960 Feb;86:233–237. doi: 10.1016/0003-9861(60)90411-2. [DOI] [PubMed] [Google Scholar]