Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1963 Mar;85(3):527–531. doi: 10.1128/jb.85.3.527-531.1963

EFFECT OF ANTIBIOTICS AND INHIBITORS ON M PROTEIN SYNTHESIS

Thomas D Brock a,1
PMCID: PMC278178  PMID: 14042928

Abstract

Brock, Thomas D. (Western Reserve University, Cleveland, Ohio). Effect of antibiotics and inhibitors on M protein synthesis. J. Bacteriol. 85:527–531. 1963.—This work extends the observations of Fox and Krampitz on M protein synthesis in nongrowing cells of streptococci. A survey of a large number of antibiotics and other potential inhibitors was made. Some substances bring about inhibition of fermentation and inhibit M protein synthesis because they deprive the cell of the energy needed for this process. A second group of substances inhibit growth at concentrations tenfold or more lower than they inhibit M protein synthesis. These are the antibiotics which inhibit synthesis of cell wall or other structures in growing cells, but do not affect protein synthesis. A third group of substances inhibit growth and M protein synthesis at the same concentration. These substances probably inhibit growth because they inhibit general protein synthesis, and are therefore specific inhibitors of protein synthesis. In this class are chloramphenicol, erythromycin, and the tetracyclines. Several other antibiotics of previously unknown mode of action are in this class. A fourth group of substances had no effect on M protein synthesis. No substances were found which inhibited M protein synthesis at a lower concentration than that which inhibited growth. M protein synthesis in nongrowing cells may be a useful model system for obtaining a detailed understanding of protein synthesis.

Full text

PDF
527

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BEN-ISHAI R. Dependence of protein synthesis on ribonucleic acid synthesis. II. Nonparticipation of preformed ribonucleic acid in protein synthesis. Biochim Biophys Acta. 1957 Dec;26(3):477–483. doi: 10.1016/0006-3002(57)90093-8. [DOI] [PubMed] [Google Scholar]
  2. BROCK T. D., BROCK M. L. Similarity in mode of action of chloramphenicol and erythromycin. Biochim Biophys Acta. 1959 May;33(1):274–275. doi: 10.1016/0006-3002(59)90535-9. [DOI] [PubMed] [Google Scholar]
  3. BROCK T. D. Magnesium binding as an explanation of the mode of action of novobiocin. Science. 1962 Apr 27;136(3513):316–317. doi: 10.1126/science.136.3513.316. [DOI] [PubMed] [Google Scholar]
  4. Brock T. D. CHLORAMPHENICOL. Bacteriol Rev. 1961 Mar;25(1):32–48. doi: 10.1128/br.25.1.32-48.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. FOX E. N., KRAMPITZ L. O. Studies on the biosynthesis of the M-protein of group A hemolytic streptococci. J Bacteriol. 1956 Apr;71(4):454–461. doi: 10.1128/jb.71.4.454-461.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. LANCEFIELD R. C., PERLMANN G. E. Preparation and properties of type-specific M antigen isolated from a group A, type 1 hemolytic streptococcus. J Exp Med. 1952 Jul;96(1):71–82. doi: 10.1084/jem.96.1.71. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. REYNOLDS P. E. Studies on the mode of action of vancomycin. Biochim Biophys Acta. 1961 Sep 16;52:403–405. doi: 10.1016/0006-3002(61)90698-9. [DOI] [PubMed] [Google Scholar]
  8. STROMINGER J. L. Mononucleotide acid anhydrides and related compounds as intermediates in metabolic reactions. Physiol Rev. 1960 Jan;40:55–111. doi: 10.1152/physrev.1960.40.1.55. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES