Abstract
Fox, Eugene N. (University of Chicago, Chicago, Ill.). Intracellular M protein of group A Streptococcus. J. Bacteriol. 85:536–540. 1963.—A heat-labile M protein antigen in protoplasts of a type 14 strain of group A Streptococcus has been demonstrated in a soluble form in the cytoplasm, and also bound to the protoplasmic membrane. When trypsinized whole cells (from which the M protein on the cell wall had been digested) or protoplasts were extracted with hot HCl, no M protein antigen was detected, although the antigen was routinely extracted from the cell walls of normal streptococci by the hot acid procedure. Various serological techniques, including the use of fluorescein-labeled type-specific antisera, were employed to demonstrate the M antigen in association with the membrane from osmotically lysed protoplasts.
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Selected References
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- FOX E. N., KRAMPITZ L. O. Studies on the biosynthesis of the M-protein of group A hemolytic streptococci. J Bacteriol. 1956 Apr;71(4):454–461. doi: 10.1128/jb.71.4.454-461.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FOX E. N. Measurement of streptococcal antigen synthesis with fluorescent antibody. Proc Soc Exp Biol Med. 1962 Mar;109:577–579. doi: 10.3181/00379727-109-27274. [DOI] [PubMed] [Google Scholar]
- FOX E. N. Peptide requirements for the synthesis of streptococcal proteins. J Biol Chem. 1961 Jan;236:166–171. [PubMed] [Google Scholar]
- FREIMER E. H., KRAUSE R. M., McCARTY M. Studies of L forms and protoplasts of group A streptococci. I. Isolation, growth, and bacteriologic characteristics. J Exp Med. 1959 Dec 1;110:853–874. doi: 10.1084/jem.110.6.853. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GOODER H., MAXTED W. R. Protoplasts of group A beta-haemolytic streptococci. Nature. 1958 Sep 20;182(4638):808–809. doi: 10.1038/182808b0. [DOI] [PubMed] [Google Scholar]
- KRAUSE R. M. Studies on the bacteriophages of hemolytic streptococci. II. Antigens released from the streptococcal cell wall by a phage-associated lysin. J Exp Med. 1958 Dec 1;108(6):803–821. doi: 10.1084/jem.108.6.803. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- MARKOVITZ A., DORFMAN A. Synthesis of capsular polysaccharide (hyaluronic acid) by protoplastmembrane preparations of group A Streptococcus. J Biol Chem. 1962 Feb;237:273–279. [PubMed] [Google Scholar]