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. 1963 Mar;85(3):595–603. doi: 10.1128/jb.85.3.595-603.1963

ISOLATION AND PROPERTIES OF AN IRON-OXIDIZING THIOBACILLUS

W E Razzell a,1, P C Trussell a
PMCID: PMC278188  PMID: 14042937

Abstract

Razzell, W. E. (British Columbia Research Council, Vancouver, Canada) and P. C. Trussell. Isolation and properties of an iron-oxidizing Thiobacillus. J. Bacteriol. 85:595–603. 1963. — An organism isolated from acidic copper-leaching waters has been shown to oxidize ferrous ions, sulfur, and metallic sulfides but exhibit peculiar responses to thiosulfate. The name Thiobacillus ferrooxidans has been used to describe it. A pH of 2.5 is optimal for growth on iron, sulfur, and metallic sulfides, but cells free from iron can be obtained from growth at pH 1.6, and sulfur cultures adjusted to pH 5.5 readily attain a pH of 1.8. A stationary cultivation procedure appears superior to percolation techniques for studying the oxidation of finely divided metallic sulfides. Concentrations of soluble copper in excess of 1 g per liter were obtained from chalcopyrite in less than 4 weeks. Chalcocite oxidation proceeded in the absence of iron. Sodium chloride inhibits iron oxidation without preventing oxidation of metallic sulfides by the organism.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BECK J. V. A ferrous-ion-oxidizing bacterium. I. Isolation and some general physiological characteristics. J Bacteriol. 1960 Apr;79:502–509. doi: 10.1128/jb.79.4.502-509.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BRALEY S. A., Sr, KINSEL N. A., LEATHEN W. W. Ferrobacillus ferrooxidans: a chemosynthetic autotrophic Bacterium. J Bacteriol. 1956 Nov;72(5):700–704. doi: 10.1128/jb.72.5.700-704.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BRYNER L. C., JAMESON A. K. Microorganisms in leaching sulfide minerals. Appl Microbiol. 1958 Jul;6(4):281–287. doi: 10.1128/am.6.4.281-287.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. COLMER A. R. Relation of the iron oxidizer, Thiobacillus ferrooxidans, to thiosulfate. J Bacteriol. 1962 Apr;83:761–765. doi: 10.1128/jb.83.4.761-765.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. COLMER A. R., TEMPLE K. L., HINKLE M. E. An iron-oxidizing bacterium from the acid drainage of some bituminous coal mines. J Bacteriol. 1950 Mar;59(3):317–328. doi: 10.1128/jb.59.3.317-328.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. KINSEL N. A. New sulfur oxidizing iron bacterium: Ferrobacillus sulfooxidans sp. n. J Bacteriol. 1960 Nov;80:628–632. doi: 10.1128/jb.80.5.628-632.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LEATHEN W. W., BRALEY S. A., Sr Interpretation of reactions in acid thiosulfate media. J Bacteriol. 1955 Apr;69(4):481–481. doi: 10.1128/jb.69.4.481-481.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LEATHEN W. W., BRALEY S. A., Sr, MCINTYRE L. D. The role of bacteria in the formation of acid from certain sulfuritic constituents associated with bituminous coal. II. Ferrous iron oxidizing bacteria. Appl Microbiol. 1953 Mar;1(2):65–68. doi: 10.1128/am.1.2.65-68.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LIALIKOVA N. N. Izuchenie protsessa khemosinteza u Thiobacillus ferrooxidans. Mikrobiologiia. 1958 Sep-Oct;27(5):556–559. [PubMed] [Google Scholar]
  10. Razzell W. E., Trussell P. C. Microbiological Leaching of Metallic Sulfides. Appl Microbiol. 1963 Mar;11(2):105–110. doi: 10.1128/am.11.2.105-110.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. SILVERMAN M. P., LUNDGREN D. G. Studies on the chemoautotrophic iron bacterium Ferrobacillus ferrooxidans. I. An improved medium and a harvesting procedure for securing high cell yields. J Bacteriol. 1959 May;77(5):642–647. doi: 10.1128/jb.77.5.642-647.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. SILVERMAN M. P., LUNDGREN D. G. Studies on the chemoautotrophic iron bacterium Ferrobacillus ferrooxidans. II. Manometric studies. J Bacteriol. 1959 Sep;78:326–331. doi: 10.1128/jb.78.3.326-331.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. TEMPLE K. L., COLMER A. R. The autotrophic oxidation of iron by a new bacterium, thiobacillus ferrooxidans. J Bacteriol. 1951 Nov;62(5):605–611. doi: 10.1128/jb.62.5.605-611.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. TEMPLE K. L., DELCHAMPS E. W. Autotrophic bacteria and the formation of acid in bituminous coal mines. Appl Microbiol. 1953 Sep;1(5):255–258. doi: 10.1128/am.1.5.255-258.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. VISHNIAC W., SANTER M. The thiobacilli. Bacteriol Rev. 1957 Sep;21(3):195–213. doi: 10.1128/br.21.3.195-213.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]

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