Abstract
Johnson, R. C. (Fort Detrick, Frederick, Md.) and N. D. Gary. Nutrition of Leptospira pomona. II. Fatty acid requirements. J. Bacteriol. 85:976–982. 1963.—The albumin fraction of rabbit serum, obtained by (NH4)2SO4 fractionation, supported good growth of Leptospira pomona Wickard when added to a medium containing phosphate buffer, NH4Cl, and thiamine. Extraction of the albumin fraction with ethanol and ether (3:1) resulted in a loss of its growth-supporting activity, which could be restored upon the addition of the extract or various fatty acids. The growth-supporting activity of fatty acids containing 2 to 18 carbon atoms was investigated with the extracted albumin medium. The activity of the fatty acid was found to be related to the number of carbon atoms in the molecule. Maximal growth was obtained with palmitic, heptadecanoic, stearic, and oleic acids. The amount of growth of L. pomona increased at a fixed concentration of albumin as the molecular ratio of extracted albumin to the long-chain fatty acids increased from 1:1 to 1:6. At higher ratios, growth decreased sharply. Direct utilization of fatty acids during growth of L. pomona was shown by the incorporation of palmitic acid-1-C14 or -2-C14 into cellular material. A medium composed of 0.02 m Na2HPO4-KH2PO4 buffer (pH 7.4), 0.8% extracted albumin, 4 × 10−4m palmitic acid, 10−3m NH4Cl, and 5 μg/ml of thiamine supported good growth. L. pomona was transferred ten times, and seven other serotypes were transferred five times, in this medium without any decrease in the amount of growth. Growth of L. pomona was initiated with approximately four organisms per ml.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ALEXANDER A. D., STOENNER H. G., WOOD G. E., BYRNE R. J. A new pathogenic Leptospira, not readily cultivated. J Bacteriol. 1962 Apr;83:754–760. doi: 10.1128/jb.83.4.754-760.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HELPRIN J. J., HIATT C. W. The effect of fatty acids on the respiration of Leptospira icterohemorrhagiae. J Infect Dis. 1957 Mar-Apr;100(2):136–140. doi: 10.1093/infdis/100.2.136. [DOI] [PubMed] [Google Scholar]
- JOHNSON R. C., GARY N. D. Nutrition of Leptospira pomona. 1. A chemically defined substitute for rabbit serum ultrafiltrate. J Bacteriol. 1962 Mar;83:668–672. doi: 10.1128/jb.83.3.668-672.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JOHNSON R. C., WILSON J. B. Nutrition of Leptospira pomona. J Bacteriol. 1960 Sep;80:406–411. doi: 10.1128/jb.80.3.406-411.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NIEMAN C. Influence of trace amounts of fatty acids on the growth of microorganisms. Bacteriol Rev. 1954 Jun;18(2):147–163. doi: 10.1128/br.18.2.147-163.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]
- OYAMA V. I., STEINMAN H. G., EAGLE H. The nutritional requirements of treponemata. V. A detoxified lipide as the essential growth factor supplied by crystallized serum albumin. J Bacteriol. 1953 May;65(5):609–616. doi: 10.1128/jb.65.5.609-616.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TERESI J. D., LUCK J. M. The combination of organic anions with serum albumin. VIII. Fatty acid salts. J Biol Chem. 1952 Feb;194(2):823–834. [PubMed] [Google Scholar]
- VANESELTINE W. P., STAPLES S. A. Nutritional requirements of Leptospirae. I. Studies of Leptospira pomona. J Infect Dis. 1961 May-Jun;108:262–269. doi: 10.1093/infdis/108.3.262. [DOI] [PubMed] [Google Scholar]
- VOGEL H., HUTNER S. H. Growth of leptospira in defined media. J Gen Microbiol. 1961 Oct;26:223–230. doi: 10.1099/00221287-26-2-223. [DOI] [PubMed] [Google Scholar]
- WORATZ H. Wachstumsversuche mit Fettsäuren an Leptospira canicola. Zentralbl Bakteriol Orig. 1957 Aug;169(3-4):269–274. [PubMed] [Google Scholar]