Abstract
Johnson, R. C. (Fort Detrick, Frederick, Md.) and N. D. Gary. Nutrition of Leptospira pomona. III. Calcium, magnesium, and potassium requirements. J. Bacteriol. 85:983–985. 1963.—The cation requirements for Leptospira pomona Wickard were investigated employing a basal medium prepared with 10% deionized rabbit serum and containing 10−3m NH4Cl, 5 μg/ml thiamine, and 0.01 m Na2HPO4 buffer (pH 7.4). Rabbit serum was deionized by passage through a column of Amberlite IRC-50 resin. This basal medium did not support growth when either Ca++ or Mg++ was added to it. However, when both Ca++ (3 × 10−5m) and Mg++ (5 × 10−6m) were present, growth was obtained. Although an absolute requirement for K+ could not be demonstrated, an increase in the amount of growth did occur if K+ (5 × 10−5m) was incorporated in addition to Ca++ and Mg++. When adequate concentrations of Ca++ and K+ were present with a limiting concentration of Mg++ (less than 5 × 10−7m), filamentous cells were observed.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- DOAK G. O., FREEDMAN L. D., CLARK J. W., Jr Ionic requirements of Treponema pallidum. III. Divalent ions. J Bacteriol. 1961 Dec;82:909–912. doi: 10.1128/jb.82.6.909-912.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FAINE S. Iron as a growth requirement for pathogenic Leptospira. J Gen Microbiol. 1959 Apr;20(2):246–251. doi: 10.1099/00221287-20-2-246. [DOI] [PubMed] [Google Scholar]
- JOHNSON R. C., GARY N. D. Nutrition of Leptospira pomona. 1. A chemically defined substitute for rabbit serum ultrafiltrate. J Bacteriol. 1962 Mar;83:668–672. doi: 10.1128/jb.83.3.668-672.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MACLEOD R. A., ONOFREY E. Nutrition and metabolism of marine bacteria. VI. Quantitative requirements for halides, magnesium, calcium, and iron. Can J Microbiol. 1957 Aug;3(5):753–759. doi: 10.1139/m57-085. [DOI] [PubMed] [Google Scholar]
- VOGEL H., HUTNER S. H. Growth of leptospira in defined media. J Gen Microbiol. 1961 Oct;26:223–230. doi: 10.1099/00221287-26-2-223. [DOI] [PubMed] [Google Scholar]
- WARDLAW A. C., PILLEMER L. The properdin system and immunity. V. The bactericidal activity of the properdin system. J Exp Med. 1956 May 1;103(5):553–575. doi: 10.1084/jem.103.5.553. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WEBB M. The influence of magnesium on cell division. V. The effect of magnesium on the growth of bacteria in chemically-defined media of varying complexity. J Gen Microbiol. 1951 Aug;5(3):485–495. doi: 10.1099/00221287-5-3-485. [DOI] [PubMed] [Google Scholar]
- WYATT H. V. Cation requirements of HeLa cells in tissue culture. Exp Cell Res. 1961 Feb;23:97–107. doi: 10.1016/0014-4827(61)90066-0. [DOI] [PubMed] [Google Scholar]