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. 1963 May;85(5):1114–1120. doi: 10.1128/jb.85.5.1114-1120.1963

ROLE OF FERREDOXIN IN THE METABOLISM OF MOLECULAR HYDROGEN

R C Valentine 1, R S Wolfe 1
PMCID: PMC278291  PMID: 14044002

Abstract

Valentine, R. C. (University of Illinois, Urbana) and R. S. Wolfe. Role of ferredoxin in the metabolism of molecular hydrogen. J. Bacteriol. 85:1114–1120. 1963.—The metabolism of molecular hydrogen by Clostridium pasteurianum, Micrococcus lactilyticus (Veillonella alcalescens), and several other anaerobic bacteria was studied. Oxidation of hydrogen, using several electron-accepting substrates including triphosphopyridine nucleotide, uric acid, xanthine, nitrite, and hydroxylamine, required ferredoxin in conjunction with hydrogenase. Evolution of hydrogen from pyruvate, α-ketoglutarate, hypoxanthine, and dithionite was mediated by ferredoxin. On the basis of these findings, a unitary hypothesis for biological hydrogen evolution is proposed in which ferredoxin plays a key role.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BAKER F. D., PAPISKA H. R., CAMPBELL L. L. Choline fermentation by Desulfovibrio desulfuricans. J Bacteriol. 1962 Nov;84:973–978. doi: 10.1128/jb.84.5.973-978.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barker H. A., Haas V. Butyribacterium, a New Genus of Gram-positive, Non-sporulating Anaerobic Bacteria of Intestinal Origin. J Bacteriol. 1944 Mar;47(3):301–305. doi: 10.1128/jb.47.3.301-305.1944. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Barker H. A. Streptococcus allantoicus and the Fermentation of Allantoin. J Bacteriol. 1943 Sep;46(3):251–259. doi: 10.1128/jb.46.3.251-259.1943. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bhat J. V., Barker H. A. Clostridium lacto-acetophilum Nov. Spec. and the Role of Acetic Acid in the Butyric Acid Fermentation of Lactate. J Bacteriol. 1947 Sep;54(3):381–391. doi: 10.1128/jb.54.3.381-391.1947. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. CAMPBELL L. L., Jr, FRANK H. A., HALL E. R. Studies on thermophilic sulfate reducing bacteria. I. Identification of Sporovibrio desulfuricans as Clostridium nigrificans. J Bacteriol. 1957 Apr;73(4):516–521. doi: 10.1128/jb.73.4.516-521.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. CARNAHAN J. E., MORTENSON L. E., MOWER H. F., CASTLE J. E. Nitrogen fixation in cell-free extracts of Clostridium pasteurianum. Biochim Biophys Acta. 1960 Nov 18;44:520–535. doi: 10.1016/0006-3002(60)91606-1. [DOI] [PubMed] [Google Scholar]
  7. ELSDEN S. R., LEWIS D. The production of fatty acids by a gram-negative coccus. Biochem J. 1953 Aug;55(1):183–189. doi: 10.1042/bj0550183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. FRY K. T., SAN PIETRO A. Studies on photosynthetic pyridine nucleotide reductase. Biochem Biophys Res Commun. 1962 Oct 17;9:218–221. doi: 10.1016/0006-291x(62)90061-x. [DOI] [PubMed] [Google Scholar]
  9. GEST H., PECK H. D., Jr A study of the hydrogenlyase reaction with systems derived from normal and anaerogenic coli-aerogenes bacteria. J Bacteriol. 1955 Sep;70(3):326–334. doi: 10.1128/jb.70.3.326-334.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gest H., Kamen M. D. STUDIES ON THE METABOLISM OF PHOTOSYNTHETIC BACTERIA IV. : Photochemical Production of Molecular Hydrogen by Growing Cultures of Photosynthetic Bacteria. J Bacteriol. 1949 Aug;58(2):239–245. [PMC free article] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. MORTENSON L. E., VALENTINE R. C., CARNAHAN J. E. An electron transport factor from Clostridium pasteurianum. Biochem Biophys Res Commun. 1962 Jun 4;7:448–452. doi: 10.1016/0006-291x(62)90333-9. [DOI] [PubMed] [Google Scholar]
  13. McCormick N. G., Ordal E. J., Whiteley H. R. DEGRADATION OF PYRUVATE BY MICROCOCCUS LACTILYTICUS I. : General Properties of the Formate-Exchange Reaction. J Bacteriol. 1962 Apr;83(4):887–898. doi: 10.1128/jb.83.4.887-898.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. NICHOLAS D. J., MEDINA A., JONES O. T. A nitrite reductase from Neurospora crassa. Biochim Biophys Acta. 1960 Jan 29;37:468–476. doi: 10.1016/0006-3002(60)90503-5. [DOI] [PubMed] [Google Scholar]
  15. PECK H. D., Jr, GEST H. A new procedure for assay of bacterial hydrogenases. J Bacteriol. 1956 Jan;71(1):70–80. doi: 10.1128/jb.71.1.70-80.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Peck H. D. THE ATP-DEPENDENT REDUCTION OF SULFATE WITH HYDROGEN IN EXTRACTS OF DESULFOVIBRIO DESULFURICANS. Proc Natl Acad Sci U S A. 1959 May;45(5):701–708. doi: 10.1073/pnas.45.5.701. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. TAGAWA K., ARNON D. I. Ferredoxins as electron carriers in photosynthesis and in the biological production and consumption of hydrogen gas. Nature. 1962 Aug 11;195:537–543. doi: 10.1038/195537a0. [DOI] [PubMed] [Google Scholar]
  18. VALENTINE R. C., JACKSON R. L., WOLFE R. S. Role of ferredoxin in hydrogen metabolism of Micrococcus lactilyticus. Biochem Biophys Res Commun. 1962 Jun 4;7:453–456. doi: 10.1016/0006-291x(62)90334-0. [DOI] [PubMed] [Google Scholar]
  19. Valentine R. C., Brill W. J., Wolfe R. S. ROLE OF FERREDOXIN IN PYRIDINE NUCLEOTIDE REDUCTION. Proc Natl Acad Sci U S A. 1962 Oct;48(10):1856–1860. doi: 10.1073/pnas.48.10.1856. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. WHITELEY H. R., DOUGLAS H. C. The fermentation of purines by Micrococcus lactilyticus. J Bacteriol. 1951 May;61(5):605–616. doi: 10.1128/jb.61.5.605-616.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. WHITELEY H. R., ORDAL E. J. Fermentation of alpha keto acids by Micrococcus aerogenes and Micrococcus lactilyticus. J Bacteriol. 1957 Sep;74(3):331–336. doi: 10.1128/jb.74.3.331-336.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. WOLFE R. S., O'KANE D. J. Cofactors of the phosphoroclastic reaction of Clostridium butyricum. J Biol Chem. 1953 Dec;205(2):755–765. [PubMed] [Google Scholar]

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