Abstract
Ralston, Doris J. (University of California, Berkeley). Staphylococcal sensitization: specific biological effects of phage K on the bacterial cell wall in lysis-from-without. J. Bacteriol. 85:1185–1193. 1963.—Phage K, shown previously to sensitize staphylococcal-wall mucopeptide to the action of a phage-induced enzyme, virolysin, was found to act in a specific manner in that its sensitizing effects were restricted to chemical linkages affected by three staphylococcal lysins. These caused an immediate lysis, whereas egg-white lysozyme, which could also digest the wall mucopeptide, exerted variable effects, even when in the absence of phage it produced some lysis. Evidence was presented that the K1 normal cell autolysin and the K phage virolysin could act synergistically with lysozyme on phage-sensitized cells, and that any effects observed with lysozyme were due to the simultaneous presence of trace amounts of these staphylococcal lysins. None of a series of lysozymelike agents from sea urchins, marine sepunculids, and from rabbit peritoneal histiocytes caused accelerated lysis of phage-sensitized cells, although like lysozyme they showed a slow lysis of phage-free living cells. Other enzymes which did not reduce the turbidity of sensitized cells included agents specific for intracellular components (proteins, lipids, nucleic acids), and enzymes, as decarboxylase, alkaline phosphatase, d-amino oxidase, and hyaluronidase. These results suggested that the main effects of the phage in sensitization were limited to areas of the cell wall involved in protection against the action of the staphylococcal lysins.
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Selected References
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- JANCZURA E., PERKINS H. R., ROGERS H. J. Teichuronic acid: a mucopolysaccharide present in wall preparations from vegetative cells of Bacillus subtilis. Biochem J. 1961 Jul;80:82–93. doi: 10.1042/bj0800082. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PARK J. T., HANCOCK R. A fractionation procedure for studies of the synthesis of cell-wall mucopeptide and of other polymers in cells of Staphylococcus aureus. J Gen Microbiol. 1960 Feb;22:249–258. doi: 10.1099/00221287-22-1-249. [DOI] [PubMed] [Google Scholar]
- PERKINS H. R. Substances reacting as hexosamine and as N-acetylhexosamine liberated from bacterial cell walls by lysozyme. Biochem J. 1960 Jan;74:186–192. doi: 10.1042/bj0740186. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RALSTON D. J., BAER B. S., ELBERG S. S. Lysis of brucellae by the combined action of glycine and a lysozyme-like agent from rabbit monocytes. J Bacteriol. 1961 Sep;82:342–353. doi: 10.1128/jb.82.3.342-353.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RALSTON D. J., BAER B. S., LIEBERMAN M., KRUEGER A. P. Lysis from without of S. aureus K1 by the combined action of phage and virolysin. J Gen Physiol. 1957 Nov 20;41(2):343–358. doi: 10.1085/jgp.41.2.343. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RALSTON D. J., BAER B. S., LIEBERMAN M., KRUEGER A. P. Virolysin: a virus-induced lysin from staphylococcal phage lysates. Proc Soc Exp Biol Med. 1955 Aug;89(4):502–507. doi: 10.3181/00379727-89-21859. [DOI] [PubMed] [Google Scholar]
- RALSTON D. J., BAER B., LIEBERMAN M., KRUEGER A. P. Virolysin, a virus-induced lysin: its appearance and function in phage-infected staphylococci. J Gen Microbiol. 1961 Mar;24:313–325. doi: 10.1099/00221287-24-3-313. [DOI] [PubMed] [Google Scholar]
- RALSTON D. J., LIEBERMAN M., BAER B., KRUEGER A. P. Staphylococcal virolysin, a phage-induced lysin; its differentiation from the autolysis of normal cells. J Gen Physiol. 1957 May 20;40(5):791–807. doi: 10.1085/jgp.40.5.791. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RICHMOND M. H. Lytic enzymes of Staphylococcus aureus 524. Biochim Biophys Acta. 1959 Feb;31(2):564–565. doi: 10.1016/0006-3002(59)90041-1. [DOI] [PubMed] [Google Scholar]
- Ralston D. J., Baer B. IMPROVED METHOD OF PHAGE ASSAY BY A PLAQUE COUNT METHOD ON MICROSCOPE SLIDES IN TRAYS. J Bacteriol. 1960 May;79(5):758–759. doi: 10.1128/jb.79.5.758-759.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SALTON M. R., GHUYSEN J. M. The structure of di- and tetrasaccharides released from cell walls by lysozyme and Streptomyces F 1 enzyme and the beta(1 to 4) N-acetylhexos-aminidase activity of these enzymes. Biochim Biophys Acta. 1959 Dec;36:552–554. doi: 10.1016/0006-3002(59)90205-7. [DOI] [PubMed] [Google Scholar]
- WORK E. The mucopeptides of bacterial cell walls. A review. J Gen Microbiol. 1961 Jun;25:169–189. doi: 10.1099/00221287-25-2-167. [DOI] [PubMed] [Google Scholar]