Table 3.
Effect of ligand binding on Cu-P(Cu (II)-1, 10-phenanthroline) stimulated disulfide bond formation1
| Receptor | No treatment | Cu -P2 | α, Cu-P3 | A, Cu-P4 | |
|---|---|---|---|---|---|
| FT-HT-Xa | 15.0 ± 4.4 | 12.0 ± 0.1 | 13.1 ± 0.2 | 12.2 ± 0.1 | |
| TM1 | L64C | 15.8 ± 0.1 | 11.3 ± 0.4 | 14.6 ± 1.0 | 13.6 ± 0.8 |
| T65C | 16.2 ± 0.1 | 14.4 ± 0.1 | 15.9 ± 0.3 | 12.8 ± 0.3 | |
| L66C | 12.3 ± 0.4 | 11.9 ± 1.0 | 13.2 ± 0.4 | 11.9 ± 0.8 | |
| I67C | 19.3 ± 4.5 | 17.0 ± 0.1 | 17.4 ± 4.1 | 18.0 ± 2.0 | |
| V68C | 28.5 ± 3.2 | 99.2 ± 0.1 | 98.3 ± 0.8 | 98.8 ± 0.1 | |
| M69C | 17.7 ± 6.9 | 14.2 ± 5.0 | 17.0 ± 6.4 | 28.9 ± 10.2 | |
| TM7 | T278C | 15.1 ± 0.8 | 65.8 ± 8.8 | 12.5 ± 0.1 | 12.4 ± 0.1 |
| T279C | 17.3 ± 0.3 | 10.1 ± 2.3 | 15.8 ± 0.2 | 10.9 ± 2.7 | |
| V280C | 15.3 ± 2.1 | 14.7 ± 1.5 | 16.3 ± 0.6 | 12.4 ± 0.1 | |
| A281C | 16.8 ± 0.5 | 11.9 ± 0.6 | 14.3 ± 1.5 | 11.3 ± 0.1 | |
| T282C | 11.7 ± 0.3 | 15.4 ± 0.1 | 12.0 ± 0.8 | 11.7 ± 0.1 | |
| L283C | 16.6 ± 0.4 | 15.7 ± 0.2 | 13.3 ± 0.1 | 10.7 ± 3.5 | |
| L284C | 15.2 ± 0.1 | 12.0 ± 0.2 | 17.7 ± 3.0 | 15.3 ± 0.1 | |
| A285C | 16.5 ± 0.8 | 53.5 ± 8.3 | 16.5 ± 2.1 | 17.7 ± 0.5 | |
| V286C | 15.6 ± 0.4 | 17.0 ± 0.3 | 13.2 ± 2.0 | 15.9 ± 0.4 | |
| L287C | 16.0 ± 0.1 | 14.3 ± 1.3 | 11.3 ± 1.7 | 16.0 ± 1.8 | |
| S288C | 14.7 ± 1.0 | 14.7 ± 1.5 | 11.5 ± 0.6 | 12.6 ± 0.2 | |
| L289C | 26.8 ± 2.0 | 61.0 ± 9.8 | 50.2 ± 12.9 | 40.9 ± 8.2 | |
| L291C | 31.8 ± 0.4 | 66.4 ± 0.1 | 52.0 ± 8.8 | 44.5 ± 5.6 | |
| S292C | 30.2 ± 8.3 | 82.3 ± 0.4 | 75.5 ± 11.4 | 65.9 ± 0.2 | |
| S293C | 21.1 ± 0.7 | 70.2 ± 5.3 | 38.5 ± 8.3 | 44.0 ± 0.1 | |
| M294C | 27.0 ± 7.6 | 75.6 ± 1.0 | 57.8 ± 0.7 | 50.8 ± 1.0 | |
| W295C | 15.7 ± 0.2 | 95.2 ± 0.7 | 76.2 ± 0.6 | 86.3 ± 0.1 | |
| A296C | 14.8 ± 2.1 | 91.1 ± 1.0 | 73.6 ± 1.5 | 82.1 ± 0.8 | |
1
The intensity of the monomer and dimer signal was measured by densitometry and the ratio of the signals was determined. The percentage of dimer was calculated as [Dimer/(Dimer+Monomer) ×100].
2
Cu-P = Cu-P(Cu (II)-1, 10-phenanthroline) treatment.
3
α, Cu-P = membranes treated with alpha-factor and Cu-P(Cu (II)-1, 10-phenanthroline).
4
A,Cu-P = membranes treated with antagonist and Cu-P(Cu (II)-1, 10-phenanthroline).