Abstract
Smith, Rodney F. (University of Maryland, Baltimore), Donald E. Shay, and Norman J. Doorenbos. Antimicrobial action of nitrogen-containing steroids. J. Bacteriol. 85:1295–1299. 1963.—A new group of 16 synthetic nitrogen-containing steroids have been tested against a variety of microorganisms for antimicrobial properties. The gradient plate screening method, serial dilution, and dry weight techniques were used in the studies. The organisms tested consisted of 14 gram-negative bacteria, 10 gram-positive bacteria, 2 actinomycetes, 7 yeasts, and 8 molds. Inhibitory properties were found to be specific and potent in four compounds, with inhibitory concentrations as low as 0.37 μg/ml. Three of the active steroids are 4-aza cholestanes and one is a 4-nor-3,5-secocholestane amide. Sensitivity to the compounds was greatest in the gram-positive bacteria, followed by the yeasts and molds. The gram-negative bacteria were not inhibited. All 16 steroids interfered to some extent with pigmentation in Serratia marcescens but not with pigment production in Pseudomonas aeruginosa. In a few instances, some of the molds were stimulated by the steroids at a concentration of 250 μg/ml.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- CASAS-CAMPILLO C., BALANDRANO D., GALARZA A. Steroids. 159. Antimicrobial properties of 21,21-dimethoxy progesterone and other progesterone analogues. J Bacteriol. 1961 Mar;81:366–375. doi: 10.1128/jb.81.3.366-375.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DOORENBOS N. J., DORN C. P., Jr Steroids. VI. Thiazoloandrostanes. J Pharm Sci. 1961 Mar;50:271–271. doi: 10.1002/jps.2600500326. [DOI] [PubMed] [Google Scholar]
- DOORENBOS N. J., SINGH H. Steroids. X. Preparation of azasteroids from cholestenone and progesterone. J Pharm Sci. 1962 May;51:418–420. doi: 10.1002/jps.2600510505. [DOI] [PubMed] [Google Scholar]
- Glassman H. N. SURFACE ACTIVE AGENTS AND THEIR APPLICATION IN BACTERIOLOGY. Bacteriol Rev. 1948 Jun;12(2):105–148. doi: 10.1128/br.12.2.105-148.1948. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HAVRANEK R. E., DOORENBOS N. J. Steroids. I. Synthesis of steroid nitrogen mustards. J Am Pharm Assoc Am Pharm Assoc. 1960 May;49:328–329. [PubMed] [Google Scholar]
- KULL F. C., CASTELLANO G. A., MAYER R. L. The in vitro antimicrobial activities of certain amino ateroids. J Invest Dermatol. 1953 Oct;21(4):227–228. doi: 10.1038/jid.1953.94. [DOI] [PubMed] [Google Scholar]
- LESTER G., HECHTER O. Effect of deoxycorticosterone on the growth of microorganisms. J Bacteriol. 1958 Oct;76(4):365–367. doi: 10.1128/jb.76.4.365-367.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Raab W. Antibacterial Action of Phenanthrene-related Substances. Science. 1946 May 31;103(2683):670–671. doi: 10.1126/science.103.2683.670. [DOI] [PubMed] [Google Scholar]
- Squire E. W., Squire E. N. Steroid Effect upon Bacterial Growth. J Bacteriol. 1948 May;55(5):766–767. [PMC free article] [PubMed] [Google Scholar]