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SufA interaction with apo and Fe-S holo forms of the SufBCD complex. His6-SufA was bound to a Ni2+-NTA column. Next, equal amounts of apo-SufBCD or Fe-S holo-SufBCD were passed over the His6-SufA-Ni2+-NTA column. After washing, all proteins bound to the column were eluted with high imidazole. All chromatography steps were carried out under strictly anaerobic conditions. Equal volumes of the elution fractions were then analyzed for protein content by SDS-PAGE.
