Abstract
Pittard, James (Yale University, New Haven, Conn.), John S. Loutit, and Edward A. Adelberg. Gene transfer by F′ strains of Escherichia coli K-12. I. Delay in initiation of chromosome transfer. J. Bacteriol. 85:1394–1401. 1963.—Two different F′ strains were found to transfer both F-merogenote and chromosomal markers to recipient strains. When zygotes having received different chromosomal markers from the male were selected, a high percentage of them were found to have also received F-merogenote markers under conditions in which the mating of more than one male cell with a single female cell was excluded. An analysis of the kinetics of zygote formation revealed that chromosomal markers began to be transferred from F′ strains 8 to 10 min later than from their analogous Hfr strains. The rate of chromosome transfer is the same from both F′ and Hfr strains, and the difference observed in kinetic experiments was shown not to be due to any difference between the sequence of chromosomal genes in the F′ and Hfr strains. F′ strains and Hfr strains showed no difference in ability to form specific pairs with female recipients shortly after mixing. The observed delay in the transfer of chromosomal markers from F′ cells thus means that, after specific pair formation, F′ cells take 8 to 10 min longer than Hfr cells to initiate chromosome transfer.
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- ADELBERG E. A., BURNS S. N. Genetic variation in the sex factor of Escherichia coli. J Bacteriol. 1960 Mar;79:321–330. doi: 10.1128/jb.79.3.321-330.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ADELBERG E. A., UMBARGER H. E. Isoleucine and valine metabolism in Escherichia coli. V. alpha-Ketoisovaleric acid accumulation. J Biol Chem. 1953 Nov;205(1):475–482. [PubMed] [Google Scholar]
- CAVALLI L. L., LEDERBERG J., LEDERBERG E. M. An infective factor controlling sex compatibility in Bacterium coli. J Gen Microbiol. 1953 Feb;8(1):89–103. doi: 10.1099/00221287-8-1-89. [DOI] [PubMed] [Google Scholar]
- CLARK A. J., ADELBERG E. A. Bacterial conjugation. Annu Rev Microbiol. 1962;16:289–319. doi: 10.1146/annurev.mi.16.100162.001445. [DOI] [PubMed] [Google Scholar]
- Dewitt S K, Adelberg E A. The Occurrence of a Genetic Transposition in a Strain of Escherichia Coli. Genetics. 1962 May;47(5):577–585. doi: 10.1093/genetics/47.5.577. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JACOB F., ADELBERG E. A. Transfert de caractéres génétiques par incorporation au facteur sexuel d'Escherichia coli. C R Hebd Seances Acad Sci. 1959 Jul 6;249(1):189–191. [PubMed] [Google Scholar]
- MATNEY T. S., ACHENBACH N. E. A comment on the fertility of F2 donor types of Escherichia coli K12. Biochem Biophys Res Commun. 1962 Oct 17;9:285–287. doi: 10.1016/0006-291x(62)90075-x. [DOI] [PubMed] [Google Scholar]
- PITTARD J., ADELBERG E. A. GENE TRANSFER BY F' STRAINS OF ESCHERICHIA COLI K-12. II. INTERACTION BETWEEN F-MEROGENOTE AND CHROMOSOME DURING TRANSFER. J Bacteriol. 1963 Jun;85:1402–1408. doi: 10.1128/jb.85.6.1402-1408.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Taylor A L, Adelberg E A. Linkage Analysis with Very High Frequency Males of Escherichia Coli. Genetics. 1960 Sep;45(9):1233–1243. doi: 10.1093/genetics/45.9.1233. [DOI] [PMC free article] [PubMed] [Google Scholar]