Abstract
Alford, John A. (U.S. Department of Agriculture, Beltsville, Md.) and David A. Pierce. Production of lipase by Pseudomonas fragi in a synthetic medium. J. Bacteriol. 86:24–29. 1963.—The growth and production of lipase by Pseudomonas fragi in 41 dehydrated media were determined. From a chemical analysis of the peptone medium which gave the maximal lipase production, numerous synthetic media were prepared. Good cell growth, but no lipase production, was obtained on a buffered ammonium sulfate-glucose medium. The addition of arginine, lysine, aspartic acid, and glutamic acid to this medium gave lipase production approaching that obtained in the peptone medium. Omission of the glucose had little effect on growth, but no lipase were produced. Sterilization of the glucose with either the amino acids or phosphate buffer had no effect on growth, but sharply reduced lipase production. Gas chromatographic analysis of the fatty acids liberated from lard, corn oil, and coconut oil indicated that medium composition had no effect on enzyme specificity. P. fluorescens produced very little lipase in any of the synthetic media examined.
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Selected References
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- CASTANEDA-AGULLO M. Studies on the biosynthesis of extracellular proteases by bacteria. I. Serratia marcescens, synthetic and gelatin media. J Gen Physiol. 1956 Jan 20;39(3):369–375. doi: 10.1085/jgp.39.3.369. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CUTCHINS E. C., DOETSCH R. N., PELCZAR M. J., Jr The influence of medium composition on the production of bacterial lipase. J Bacteriol. 1952 Feb;63(2):269–272. doi: 10.1128/jb.63.2.269-272.1952. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HALVORSON H. O. The induced synthesis of proteins. Adv Enzymol Relat Subj Biochem. 1960;22:99–156. doi: 10.1002/9780470122679.ch3. [DOI] [PubMed] [Google Scholar]
- HARTMAN R. E., ZIMMERMAN L. N., RABIN R. Proteinase biosynthesis by Streptococcus liquefaciens. II. Purine, pyrimidine, and vitamin requirements. Can J Microbiol. 1957 Jun;3(4):553–558. doi: 10.1139/m57-060. [DOI] [PubMed] [Google Scholar]
- JAYKO L. G., LICHSTEIN H. C. Nutritional factors concerned with growth and lecithinase production by Clostridium perfringens. J Infect Dis. 1959 Mar-Apr;104(2):142–151. doi: 10.1093/infdis/104.2.142. [DOI] [PubMed] [Google Scholar]